Lightning-Link® Rapid FluoProbes647H Antibody Labeling Kit

Flow Cytometry: Lightning-Link Rapid FluoProbes 647H Antibody Labeling Kit [362-0030] - Mouse anti-human CD3 was conjugated with FluoProbe® 647H using Lightning-Link® Rapid kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line - negative control; red line - positive staining).

Lightning-Link Rapid FluoProbes 647H Antibody Labeling Kit [362-0030]

• Conjugate: FluoProbes 647H
• Format: FluoProbes 647H (A=653, E=674)
• Kit Type: Antibody Labeling Kit

Lightning-Link® Rapid FluoProbes647H Antibody Labeling Kit Summary

• Description: Lightning-Link Rapid is an innovative technology that enables direct labeling of proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits (See protocol for further information).
  
  The easy-to-use, one step procedure allows researchers to covalently label biomolecules with only 30 seconds hands-on time; furthermore conjugates are ready to use in less than twenty minutes.
  
  The researcher simply pipettes the biomolecule into a vial of lyophilized mixture containing the label of interest and incubates (for more details please watch the video below).
  
  

  Features	Benefits
  Quick and easy to use	Save time, no special knowledge required
  No separation steps	100% recovery - no antibody/protein loss
  Can be used in a wide range of applications	Flexible
  Freeze dried	Ships at ambient temperature, long shelf-life
  Fully scalable (10 ug to 1 g or more)	Easy transfer from R&D to manufacturing
  Stringently QC tested	Consistent high quality, excellent batch-to-batch reproducibility
  Large number of labels available	Experimental flexibility
  Reliable: nearly 300 references	Successfully used in many fields of research

  
  
  FluoProbes647H is one of a new generation of fluorescent labels designed to label biomolecules. It has a strong absorption at 650nm, high fluorescence at 684nm (extinction coefficient 2.5 x105 cm-1M-1) and high quantum yield.
  
  Learn more about Lightning-Link™ Conjugation Kits by reading FAQs
  
  For more information please check out these useful links!
  Antibody Labeling Guide
  Antibody Conjugation Illustrated Assay
• Kit Type: Antibody Labeling Kit

Applications/Dilutions

• Application Notes: By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, LightningLink technology can be used to label both small (e.g. 10 ug) and large quantities of primary antibodies with ease. Batch-to-batch variation upon scale up is minimal as the process is so simple, and recoveries are always 100%. This kit is supplied with 3 vials, each suitable for labeling up to 20 ug of antibody.

Packaging, Storage & Formulations

• Storage: Store at -20C.

Notes

This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

Background

Lightning-Link® Rapid Antibody Labeling kits

Allows direct labeling of antibodies, proteins, peptides or other biomolecules. Procedure is completed in seconds, and used within minutes (less than 20 minutes). Process generates conjugates identical, or better than, traditional conjugation procedures. 100 percent recovery.

For more information please check out these useful links!

Antibody Labeling Guide

Purifaction Antibody Guide

Lightning-Link® Demonstration Video

Fluorescent Labels Poster

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Lightning-Link (R) Rapid FluoProbes647H Kit (362-0030)(2)

Publications using 362-0030

• Aravantinou M, Frank I, Arrode-Bruses G et al. A model of genital herpes simplex virus Type 1 infection in Rhesus Macaques. J Med Primatol. 2017-01-01 [PMID: 28748667]
• Kakhlon O, Glickstein H, Feinstein N et al. Polyglucosan neurotoxicity caused by glycogen branching enzyme deficiency can be reversed by inhibition of glycogen synthase. J Neurochem 2013-04-18 [PMID: 23607684]