Lightning-Link® Rapid Alexa Fluor 647 Antibody Labeling Kit

Lightning-Link (R) Rapid Alexa Fluor 647 Antibody Labeling Kit [336-0005] - Scans of the Absorbance and Fluorescence Emission of a Lightning-Link® Rapid Alexa Fluor® 647 Conjugate

• Conjugate: Alexa Fluor 647
• Format: Alexa Fluor 647 (A=650, E=668)
• Kit Type: Antibody Labeling Kit

Lightning-Link® Rapid Alexa Fluor 647 Antibody Labeling Kit Summary

• Description: Lightning-Link Rapid Antibody Labeling kits enable direct labeling of antibodies, proteins, peptides or other biomolecules (see protocol for further information) and allow conjugations to be set up in seconds, and used within minutes (less than 20 minutes).
  
  Our Lightning-Link Rapid technology can be used to label small quantities of antibody with 100% recovery. The researcher simply pipettes the antibody or other biomolecule into a vial of lyophilized mixture containing the label of interest and incubates the reaction for only 15 minutes (for more details please watch the video below). As there are no desalting or dialysis steps that commonly interrupt traditional protein labeling procedures.
  
  

  Features	Benefits
  Quick and easy to use	Save time, no special knowledge required
  No separation steps	100% recovery - no antibody/protein loss
  Can be used in a wide range of applications	Flexible
  Freeze dried	Ships at ambient temperature, long shelf-life
  Fully scalable (10 ug to 1 g or more)	Easy transfer from R&D to manufacturing
  Stringently QC tested	Consistent high quality, excellent batch-to-batch reproducibility
  Large number of labels available	Experimental flexibility
  Reliable: nearly 300 references	Successfully used in many fields of research

  
  
  Learn more about Lightning-Link™ Conjugation Kits by reading FAQs
  
  For more information please check out these useful links!
  Antibody Labeling Guide
  Antibody Conjugation Illustrated Assay
• Kit Type: Antibody Labeling Kit

Applications/Dilutions

• Application Notes: By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, LightningLink technology can be used to label both small (e.g. 10 ug) and large quantities of primary antibodies with ease. Batch-to-batch variation upon scale up is minimal as the process is so simple, and recoveries are always 100%.

Packaging, Storage & Formulations

• Storage: Store at -20C.

Notes

This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Lightning-Link (R) Rapid Alexa Fluor 647 Kit (336-0005)(5)

Publications using 336-0005

• Alzamareh D, Meednu N, Nandedkar-Kulkarni N et al. Interferon activation in bone marrow long-lived plasma cells in systemic lupus erythematosus Frontiers in Immunology 2025-01-10 [PMID: 39867907]
• Kayagaki N, Stowe IB, Alegre K et al. Inhibiting membrane rupture with NINJ1 antibodies limits tissue injury Nature 2023-05-17 [PMID: 37196676]
• Wanner N, Barnhart J, Apostolakis N Et al. Using the Autofluorescence Finder on the Sony ID7000(TM) Spectral Cell Analyzer to Identify and Unmix Multiple Highly Autofluorescent Murine Lung Populations Front Bioeng Biotechnol 2022-04-04 [PMID: 35372303]
  
  Details:
  Citation using the Alexa Fluor 532 version of this antibody.
• Alameh MG, TombAcz I, Bettini E Et al. Lipid nanoparticles enhance the efficacy of mRNA and protein subunit vaccines by inducing robust T follicular helper cell and humoral responses Immunity 2021-11-01 [PMID: 34852217]
• Awasthi S, Knox JJ, Desmond A Et al. Trivalent nucleoside-modified mRNA vaccine yields durable memory B cell protection against genital herpes in preclinical models The Journal of clinical investigation 2021-10-07 [PMID: 34618692]