Lightning-Link® Glucose Oxidase Antibody Labeling Kit Summary
Lightning-Link antibody labeling kits enable the direct labeling of antibodies, proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits (See protocol for further information).
Our glucose oxidase antibody labeling kit enables the direct conjugation of glucose oxidase to any biomolecule with an available amine group. The researcher simply pipettes their antibody or other biomolecule into the vial of Lightning-Link label and incubates for 3 hours.
|Quick and easy to use||Save time, no special knowledge required|
|No separation steps||100% recovery - no antibody/protein loss|
|Can be used in a wide range of applications||Flexible|
|Freeze dried||Ships at ambient temperature, long shelf-life|
|Fully scalable (10 ug to 1 g or more)||Easy transfer from R&D to manufacturing|
|Stringently QC tested||Consistent high quality, excellent batch-to-batch reproducibility|
|Large number of labels available ||Experimental flexibility|
|Reliable: nearly 300 references||Successfully used in many fields of research|
Glucose oxidase is an enzyme produced and purified from Aspergillus niger which catalyses the oxidation of glucose with the release of hydrogen peroxide. It's molecular weight is 80kDa, but it exists as a dimer of 160kDa Measurement of glucose by GOx is used in the food industry, in fermentation and, most importantly, as the basis of biosensors for the diagnosis of diabetes.
Antibody Labeling Kit
By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, LightningLink technology can be used to label both small (e.g. 10 ug) and large quantities of primary antibodies with ease. Batch-to-batch variation upon scale up is minimal as the process is so simple, and recoveries are always 100%. This kit is supplied with 3 vials, each suitable for labeling up to 100 ug of antibody.
Packaging, Storage & Formulations
- 1 or 3 glass vial(s) of Lightning-Link mix
- 1 vial of LL-Modifier reagent
- 1 vial of LL-Quencher reagent
Learn more about Lightning-Link™ Conjugation Kits by reading FAQs
For more information please check out these useful links! Antibody Labeling Guide Antibody Conjugation Illustrated Assay
This product is manufactured by Abcam and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact email@example.com with questions.
Easy Glucose Oxidase labeling of your antibody with 30 seconds hands-on time. Glucose oxidase (GOx) is an enzyme produced and purified from Aspergillus niger which catalyses the oxidation of glucose with the release of hydrogen peroxide. The molecular weight of Glucose oxidase is 80kDa, but it exists as a dimer of 160kDa. The Lightning-Link conjugation system represents a quantum leap forward in conjugation technology. It allows you to make antibody conjugates with minimal hands-on time - less than 30 seconds. Lightning-Link simplifies immunoassay techniques, such as western blotting, ELISA and immunohistochemistry, by eliminating secondary reagents and cutting the number of incubation and wash steps. Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality conjugates. As the procedure is not interrupted by desalting steps, trial conjugates can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed
for 6 months from date of receipt.
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