Lightning-Link® B-Phycoerythrin Antibody Labeling Kit

Flow Cytometry: Lightning-Link B-Phycoerythrin Antibody Labeling Kit [716-0005] - Mouse anti-human CD3 was conjugated with B Phycoerythrin using a Lightning-Link kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line - negative control; red line - positive staining).

• Conjugate: B-Phycoerythrin
• Format: B-Phycoerythrin (A=545;563, E=578)
• Kit Type: Antibody Labeling Kit

Lightning-Link® B-Phycoerythrin Antibody Labeling Kit Summary

• Description: Lightning-Link antibody labeling kits enable the direct labeling of antibodies, proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits (See protocol for further information).
  
  Our B-PE antibody labeling kit enables the direct conjugation of B-PE to any biomolecule with an available amine group. The researcher simply pipettes the antibody or other biomolecule into the vial of Lightning-Link R-PE and incubates for 3 hours.
  
  

  Features	Benefits
  Quick and easy to use	Save time, no special knowledge required
  No separation steps	100% recovery - no antibody/protein loss
  Can be used in a wide range of applications	Flexible
  Freeze dried	Ships at ambient temperature, long shelf-life
  Fully scalable (10 ug to 1 g or more)	Easy transfer from R&D to manufacturing
  Stringently QC tested	Consistent high quality, excellent batch-to-batch reproducibility
  Large number of labels available	Experimental flexibility
  Reliable: nearly 300 references	Successfully used in many fields of research

  
  
  BPE is a fluorescent protein from the phycobiliprotein family from the cyanbacteria and eukaryotic algae. It has a strong absorption peak at about 546 nm and has an emission value of 580 nm.
  
  B-Phycoerythrin is often thought to be less "sticky" than R-Phycoerythrin therefore BPE conjugates tend to produce less background signal from non-specific binding in certain applications
• Kit Type: Antibody Labeling Kit

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, LightningLink technology can be used to label both small (e.g. 10 ug) and large quantities of primary antibodies with ease. Batch-to-batch variation upon scale up is minimal as the process is so simple, and recoveries are always 100%. This kit can be used to label up to 60 ug of antibody, and is supplied in one vial.

Packaging, Storage & Formulations

• Storage: Store at -20C.

Notes

Learn more about Lightning-Link™ Conjugation Kits by reading FAQs

For more information please check out these useful links!
Antibody Labeling Guide
Antibody Conjugation Illustrated Assay

This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

Background

Easy B-PE labeling of your antibody with 30 seconds hands-on time. B-Phycoerythrin (used in this kit) is a red protein from the light-harvesting phycobiliprotein family, isolated from cyanobacteria, red algae and cryptomonads. Due to its different subunit composition, B-PE has a slightly different absorbance and emission spectrum to RPE. Its absorption peak is at 545 nm and its emission peak is at 575. The Lightning-Link conjugation system represents a quantum leap forward in conjugation technology. It allows you to make antibody conjugates with minimal hands-on time - less than 30 seconds. Lightning-Link simplifies immunoassay techniques, such as western blotting, ELISA and immunohistochemistry, by eliminating secondary reagents and cutting the number of incubation and wash steps.

Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality conjugates. As the procedure is not interrupted by desalting steps, trial conjugates can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Lightning-Link (R) B-Phycoerythrin Kit (716-0005)(6)

Publications using 716-0005

• van der Vlist EJ, Nolte-'t Hoen EN, Stoorvogel W et al. Fluorescent labeling of nano-sized vesicles released by cells and subsequent quantitative and qualitative analysis by high-resolution flow cytometry Nature Protocols 2012-01-01 [PMID: 22722367] (FLOW)
• Hoen EN, van der Vlist EJ, Aalberts M et al. Quantitative and qualitative flow cytometric analysis of nanosized cell-derived membrane vesicles Nanomedicine: Nanotechnology, Biology and Medicine 2011-01-01 [PMID: 22024193] (FLOW)
• Al-Dujaili EA, Mullins LJ, Bailey MA, Kenyon CJ. Development of a Highly Sensitive ELISA for Aldosterone in Mouse Urine: Validation in Physiological Pathophysiological States of Aldosterone Excess Depletion - . Steroids. 2008-01-01 [PMID: 19162057]
• Bao S, Wu Q, Li Z et al. Targeting Cancer Stem Cells through L1CAM Suppresses Glioma Growth. Cancer Res 2008-01-01 [PMID: 18676824]
• Marr AK, Jenssen H, Moniri MR et al. Bovine lactoferrin and lactoferricin interfere with intracellular trafficking of Herpes simplex virus-1. Biochemie 2009-01-01 [PMID: 18573311]
• Velappan N, Clements J, Kiss C et al. Fluorescence linked immunosorbant assays using microtiter plates. J Immunol Methods. 2013-01-01 [PMID: 18514691]

FAQs for Lightning-Link (R) B-Phycoerythrin Kit (716-0005). (Showing 1 - 1 of 1 FAQs).

1. Can I use your lightning-link kits to label other proteins rather than antibodies?
   • Yes, the kits work by using free amines, so as long as you have that the kit should work.