Flow Cytometry: Lightning-Link B-Phycoerythrin Antibody Labeling Kit [716-0005] - Mouse anti-human CD3 was conjugated with B Phycoerythrin using a Lightning-Link kit. The conjugated antibody was then used to stain ...read more
Lightning-Link antibody labeling kits enable the direct labeling of antibodies, proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits (See protocol for further information).
Our B-PE antibody labeling kit enables the direct conjugation of B-PE to any biomolecule with an available amine group. The researcher simply pipettes the antibody or other biomolecule into the vial of Lightning-Link R-PE and incubates for 3 hours.
Quick and easy to use
Save time, no special knowledge required
No separation steps
100% recovery - no antibody/protein loss
Can be used in a wide range of applications
Ships at ambient temperature, long shelf-life
Fully scalable (10 ug to 1 g or more)
Easy transfer from R&D to manufacturing
Stringently QC tested
Consistent high quality, excellent batch-to-batch reproducibility
Large number of labels available
Reliable: nearly 300 references
Successfully used in many fields of research
BPE is a fluorescent protein from the phycobiliprotein family from the cyanbacteria and eukaryotic algae. It has a strong absorption peak at about 546 nm and has an emission value of 580 nm.
B-Phycoerythrin is often thought to be less "sticky" than R-Phycoerythrin therefore BPE conjugates tend to produce less background signal from non-specific binding in certain applications
Antibody Labeling Kit
By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, LightningLink technology can be used to label both small (e.g. 10 ug) and large quantities of primary antibodies with ease. Batch-to-batch variation upon scale up is minimal as the process is so simple, and recoveries are always 100%. This kit can be used to label up to 60 ug of antibody, and is supplied in one vial.
This product is manufactured by Abcam and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact firstname.lastname@example.org with questions.
Easy B-PE labeling of your antibody with 30 seconds hands-on time. B-Phycoerythrin (used in this kit) is a red protein from the light-harvesting phycobiliprotein family, isolated from cyanobacteria, red algae and cryptomonads. Due to its different subunit composition, B-PE has a slightly different absorbance and emission spectrum to RPE. Its absorption peak is at 545 nm and its emission peak is at 575. The Lightning-Link conjugation system represents a quantum leap forward in conjugation technology. It allows you to make antibody conjugates with minimal hands-on time - less than 30 seconds. Lightning-Link simplifies immunoassay techniques, such as western blotting, ELISA and immunohistochemistry, by eliminating secondary reagents and cutting the number of incubation and wash steps.
Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality conjugates. As the procedure is not interrupted by desalting steps, trial conjugates can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
Publications for Lightning-Link (R) B-Phycoerythrin Kit (716-0005)(6)
We have publications tested in 1 application: FLOW.