Lightning-Link® Alkaline Phosphatase Animal Free Antibody Labeling Kit

Product Discontinued

Lightning-Link® Alkaline Phosphatase Animal Free Antibody Labeling Kit Summary

• Description: Lightning-Link antibody labeling kits enable the direct labeling of antibodies, proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits (See protocol for further information).
  
  Our Lightning-Link(R) Alkaline Phosphatase AF (Animal Free) antibody labeling kit enables the direct conjugation of antibodies, proteins and peptides (or any other biomolecule with an available amine group) to alkaline phosphatase, with only 30 seconds hands-on time and with 100% recovery of materials.
  
  

  Features	Benefits
  Quick and easy to use	Save time, no special knowledge required
  No separation steps	100% recovery - no antibody/protein loss
  Can be used in a wide range of applications	Flexible
  Freeze dried	Ships at ambient temperature, long shelf-life
  Fully scalable (10 ug to 1 g or more)	Easy transfer from R&D to manufacturing
  Stringently QC tested	Consistent high quality, excellent batch-to-batch reproducibility
  Large number of labels available	Experimental flexibility
  Reliable: nearly 300 references	Successfully used in many fields of research

  
  
  Alkaline phosphatase is a hydrolyase enzyme that is frequently conjugated to antibodies for use in immunoassays. PNPP is a popular colorimetric substrate for AP, the product of the reaction, p-nitrophenol, can be read at 405 nM. Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate, MUP) may also be employed.
  
  Alkaline phosphatase AF (Animal Free Origin) catalyses the same reaction of conventional alkaline phosphatase (EC 3.1.3.1) but it is produced in a microbial host, representing an alternative to bovine alkaline phosphatase, to support scientists working with assays where AF conditions are critical, without compromising on quality, stability and accuracy.
  
  Additionally, paperwork and safety restrictions for transport and handling can be reduced as our AF alkaline phosphatase is provided BSA-free.
  
  
  
  Learn more about Lightning-Link™ Conjugation Kits by reading FAQs
  
  For more information please check out these useful links!
  Antibody Labeling Guide
  Antibody Conjugation Illustrated Assay
• Kit Type: Antibody Labeling Kit

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, Lightning-Link(R) technology can be used to label both small (e.g. 10 ug) and large quantities of primary antibodies with ease. Batch-to-batch variation upon scale up is minimal as the process is so simple, and recoveries are always 100%.

Packaging, Storage & Formulations

• Storage: Store at -20 °C.

Notes

This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.