Leucine Aminopeptidase (LAP) Assay Kit (Fluorometric)

Leucine Aminopeptidase (LAP) Activity Fluorometric Assay Kit [NBP2-59745]

Leucine Aminopeptidase (LAP) Activity Fluorometric Assay Kit [NBP2-59745]

Leucine Aminopeptidase (LAP) Activity Fluorometric Assay Kit [NBP2-59745]

Product Discontinued

Leucine Aminopeptidase (LAP) Assay Kit (Fluorometric) Summary

• Description: This kit provides a quick, sensitive and easy way for measuring total LAP activity in various samples. In this assay, LAPs hydrolyze leucine from the fluorescent probe and the amount of fluorescent probe detected is used to determine the total activity of the LAP enzymes. Detection method: Fluorescence (Ex/Em = 368/460 nm). The assay is simple to perform, high-throughput adaptable and can detect less than 0.1 mU of LAP activity.
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: K534-100)
• Kit Type: Assay Kit (Fluorometric)

Packaging, Storage & Formulations

• Storage: Store at -20C in the dark. Avoid freeze-thaw cycles.

Background

Leucine aminopeptidases (EC 3.4.11.1) (LAPs) are a diverse set of exopeptidases that catalyze the hydrolysis of leucine residues from the amino-termini of proteins or peptides. LAPs are ubiquitous enzymes present among animals, plants and prokaryotes. Previously, they were thought to typically play important roles in cell maintenance, growth and development. However, research in the recent years has identified multiple secondary functions for these enzymes in animals and microbes including transcriptional regulation and vesicle transport. Studies have implicated LAP enzymes in tumor cell proliferation, invasion and angiogenesis. Placental LAP is used as a biomarker in ovarian epithelial cancer while adipocyte-derived LAP is used as a marker of endometrial cancer cell proliferation and differentiation. LAP enzymes are also known to be involved in catabolism of oxytocin and vasopressin and insulin regulation of GLUT4 receptors in diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.