Recombinant Human LC3B2 His Protein

SDS-Page: Recombinant Human LC3B2 Protein [NBP1-99115] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human LC3B2 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-120 of Human LC3B2
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPSEKTFKQR RTFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE SEKDEDGFLY MVCASQETFG

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: MAP1LC3B2
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 16.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol, 0.1 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human LC3B2 His Protein

• ATG8G
• microtubule-associated protein 1 light chain 3 beta 2
• microtubule-associated proteins 1A/1B light chain 3 beta 2
• Microtubule-associated proteins 1A/1B light chain 3B-like protein

Background

Microtubule-associated proteins 1A/1B light chain 3 beta 2, also as known as MAP1LC3B2, belongs to the MAP1LC3 family. MAP1LC3B2 is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. This protein probably involved in formation of autophagosomal vacuoles (autophagosomes). It is expressed primarily in heart, testis, brain and skeletal muscle and testis. Recombinant human MAP1LC3B2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for LC3B2 Recombinant Protein (NBP1-99115)(1)

Publication using NBP1-99115

• Li , Wang C, Li Z et al. Nano-sized Al2O3 particle-induced autophagy reduces osteolysis in aseptic loosening of total hip arthroplasty by negative feedback regulation of RANKL expression in fibroblasts Cell Death Dis 2018-08-12 [PMID: 30082761] (ICC/IF, IF/IHC, WB, Human)

Bioinformatics

• Gene Symbol: MAP1LC3B2