L1/ORF2 Antibody

• Reactivity: Mu
• Applications: ELISA
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated

L1/ORF2 Antibody Summary

• Immunogen: This IgY fraction L1/ORF2 Antibody was prepared from eggs of chickens laid after repeated immunizations with three synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein. (Uniprot: P11369)
• Specificity: Anti-L1/ORF2 Antibody is directed against three regions within the endonuclease domain of L1 ORF2 protein. Reactivity occurs against mouse L1/ORF2 protein and is useful in determining its presence in various assays. A BLAST analysis shows reactivity with L1/ORF2 proteins from mouse sources based on 100% homology with the immunizing sequences. Cross reactivity against L1/ORF2 from other sources is unknown.
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000-1:100000
• Application Notes: This product has been tested for use in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 149.5 kDa in size corresponding to L1 protein by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

Cross reactivity against L1/ORF2 from other sources is unknown

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Notes

Chicken products cannot be exported to Canada.

This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for L1/ORF2 Antibody

• EC=2.7.7.49
• EC=3.1.21.-
• Endonuclease
• L1
• LINE-1 retrotransposable element ORF2 protein
• Long interspersed element-1
• ORF2p
• Retrovirus-related Pol polyprotein LINE-1
• Reverse transcriptase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P11369(Human)