L-Asparaginase Antibody [HRP]

Product Discontinued

L-Asparaginase Antibody [HRP] Summary

• Description: This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
• Immunogen: Anti-Asparaginase Antibody was produced by repeated immunizations with E. coli Asparaginase protein.
• Localization: Cytoplasmic and Secreted.
• Specificity: Recognises purified and partially purified Asparaginase (E. coli).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:5000
  • Western Blot 1:200-1:1000
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Reactivity Notes

Cross reactivity against Asparaginase from other tissues and species may occur but have not been tested.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Gentamicin Sulfate
• Concentration: LYOPH
• Purity: Ion exchange chromatography
• Reconstitution Instructions: Reconstitute with 100 ul deionized water.

Alternate Names for L-Asparaginase Antibody [HRP]

• AnsA
• AnsB
• Asparaginase
• Colaspase
• Cytoplasmic asparaginase I
• L ASNase I
• L ASNase II
• L asparaginase 1
• L asparaginase 2
• L asparaginase I
• L asparaginase II precursor
• L asparaginase II
• L asparagine amidohydrolase I
• L asparagine amidohydrolase II

Background

Asparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity. E.coli contains two L asparaginase isoenzymes: L asparaginase I, a low affinity enzyme located in the cytoplasm, and L asparaginase II, a high affinity secreted enzyme.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.