KRTAP2-4 Antibody (OTI3B4) - Azide and BSA Free

Western Blot: KRTAP2-4 Antibody (OTI3B4) - Azide and BSA Free [NBP2-72304] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRTAP2-4.

Immunohistochemistry: KRTAP2-4 Antibody (OTI3B4) - Azide and BSA Free [NBP2-72304] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Flow Cytometry: KRTAP2-4 Antibody (OTI3B4) - Azide and BSA Free [NBP2-72304] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by KRTAP2 antibody, and then analyzed by flow cytometry.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI3B4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

KRTAP2-4 Antibody (OTI3B4) - Azide and BSA Free Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-128 of human KRTAP2(NP_149440) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRTAP2-4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Western Blot 1:500
• Theoretical MW: 13.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for KRTAP2-4 Antibody (OTI3B4) - Azide and BSA Free

• High sulfur keratin-associated protein 2.4
• KAP2.4MGC119884
• keratin associated protein 2-4
• Keratin-associated protein 2.4
• keratin-associated protein 2-4
• KRTAP2.4
• MGC119882
• MGC119885
• MGC74790

Background

KRTAP2-4 is a member of the keratin-associated protein (KAP) family. The KAP proteins form a matrix of keratin intermediate filaments which contribute to the structure of hair fibers. KAP family members appear to have unique, family-specific amino- and carboxyl-terminal regions and are subdivided into three multi-gene families according to amino acid composition: the high sulfur, the ultrahigh sulfur, and the high tyrosine/glycine KAPs. This protein is a member of the high sulfur KAP family and the gene is localized to a cluster of KAPs at 17q12-q21.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRTAP2-4