KLHDC8A Overexpression Lysate


Western Blot: KLHDC8A Overexpression Lysate (Adult Normal) [NBL1-12329] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KLHDC8A.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

KLHDC8A Overexpression Lysate Summary

Transient overexpression lysate of kelch domain containing 8A (KLHDC8A) The lysate was created in HEK293T cells, using Plasmid ID RC206258 and based on accession number NM_018203. The protein contains a C-MYC/DDK Tag.


Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
38.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for Array



HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for KLHDC8A Overexpression Lysate

  • FLJ10748
  • kelch domain containing 8A
  • kelch domain-containing protein 8A


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Gene Symbol KLHDC8A