Klebsiella Aerogenes Antibody (73/28) [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Ba
• Applications: ELISA, ICC/IF
• Clone: 73/28
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

Klebsiella Aerogenes Antibody (73/28) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: Native Klebsiella aerogenes preparation
• Specificity: In a simple ELISA using Biogenesis test groups (BTGs) this is reactive with many serotypes of Klebsiella. Immunohistology: Has detected Klebsiella oxytoca and other serotypes in fresh isolates, including those fixed by phenol, formalin or boiling.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Bacteria. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Klebsiella Aerogenes Antibody (73/28) [CoraFluor™ 1]

• k aerogenes
• K. aerogenes

Background

Klebsiella are non-motile, rod-shaped, aerobic bacteria that possess a prominent polysaccharide capsule. Some of these bacteria produce an extracellular toxic complex that has been shown to be "lethal for and produce extensive lung pathology in mice". It is composed of 63% capsular polysaccharide, 30% lipopolysaccharide, and 7% protein; when introduced to experimental animals in sublethal doses, the animals built up immunization due to antibody production. Klebsiella pneumoniae is one of the most common Gram-negative bacteria seen by physicians worldwide.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.