Human Kidney Tissue Lysate (Adult Tumor)

• Reactivity: Hu

Human Kidney Tissue Lysate (Adult Tumor) Summary

• Description:

  Matched Tumor & Normal Tissue Lysate Set

  Diagnosis: Renal cell carcinoma
  
  Sex: Female
  
  Age: 44
  
  Grade: 3
  
  Stage: III, T_3bN₀M_x
  
  Tumor Pathology Data
  
  Location: Right Kidney
  
  Gross findings: Tumor size 10.4 cm. Cut section, tan/yellow/red, hemorrhagic and necrotic. Lymph nodes negative for metastatic carcinoma.
  
  Microscopic findings
  
  The tumor is composed of enlarged cells with oval-round nuclei, distinct nucleoli, and abundant amounts of clear or eosinophilic cytoplasm. Cellular pleomorphism is generally mild, however, in some areas severe anaplastic nuclear pleomorphism is seen. Cells are arranged in enlarged islands/nests with intervening fibrovascular septae. Extensive tumor necrosis is present. The neoplastic process does not extend through the renal capsule. Extension into the renal vein with involvement of the vascular margin of resection is seen. The non-neoplastic portion of kidney demonstrates varying degrees of glomerulosclerosis, interstitial fibrosis, and inflammation.
  
  Preparation Method
  
  Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify.
  Extraction 1: PBS, pH 7.4; 1 ug/ml Aprotinin; 1 mM NaF
  Modified RIPA Buffer: 1 mM EDTA; 1 ug/ml Pepstatin-A; 0.1% SDS; 0.25% Na deoxycholate; 1 ug/ml Leupeptin; 1 mM PMSF; 1 mM Na₃VO₄

Applications/Dilutions

• Application Notes: These lysates have not been subjected to denaturing or reducing conditions. This allows the tissue or cell lysate to be used in a variety of applications; to study protein-protein interaction, ligand binding, ELISA, immunoprecipitation, 1D and 2D gel electrophoresis, and Western blotting for the detection of specific protein targets.
  For use in 1D and 2D gel electrophoresis, the addition of a denaturing gel loading buffer with reducing agents may be required.
  Buffer requirements for performing protein-protein interaction and ligand binding studies can vary significantly from RIPA buffer and may require modifications. In most cases, tissue lysates in RIPA buffer can be used, directly in standard ELISA and immunoprecipitation assays.
  These lysates are proteomic discovery tools. Researchers should validate and optimize for individual use.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.

Lysate Details for Array

• Type: Tissue
• Life Stage: Adult
• Tissue: Kidney
• Tissue Condition: Tumor

Notes

The vial is provided with a 10% overfill. Maximum recovery can be obtained by centrifuging the vial briefly to collect any solution on the cap and tube sides.

This material has tested negative for HbsAg, HIV 1/2, and HCV. Use UNIVERSAL PRECAUTIONS when handling. Human tissue derivatives must be treated as a potentially infectious agent and disposed of appropriately

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.