Jurkat Whole Cell Lysate Summary
Whole cell lysate (denatured)
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay. The lysate was mixed in 5X Sample Buffer to become final Sample Buffer as Storage Buffer. The lysate was heated at 95C for 5 min and cooled rapidly.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
In ready-to-use Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% Glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue). Lysis Buffer (50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF).
Lysate Details for Array
Quality control test: 12.5% SDS-PAGE Stained with Coomassie Blue.
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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