jmjd-2 Activity/Inhibition Assay Kit (Colorimetric)

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jmjd-2 Assay/Inhibitor Screening Assay Kit [KA1526] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is ...read more

Product Details

Summary
Applications Func
Suitable Sample Type
Nuclear extracts or JMJD2 subtypes (JMJD2A-JMJD2F) using purified enzymes from a broad range of species such as mammals, plants, fungi, and bacteria, in a variety of forms including cultured cells and fresh tissues
Standard Curve Range
1 to 20 ug nuclear extract; 10 to 500 ng purified enzyme
Sensitivity
1 ug nuclear extract; 10 ng purified enzyme

Order Details

jmjd-2 Activity/Inhibition Assay Kit (Colorimetric) Summary

Description
Quality control test: Standard Curve JMJD2 Demethylase Activity/Inhibition Assay Kit (Colorimetric) is use for screening JMJD2 demethylase inhibitors.
Standard Curve Range
1 to 20 ug nuclear extract; 10 to 500 ng purified enzyme
Sensitivity
1 ug nuclear extract; 10 ng purified enzyme
Assay Type
Colorimetric
Sample Volume
1- 4 uL
Kit Type
Activity/Inhibition Assay Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • Functional

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. 8-Well Assay Strips (With Frame)
  2. Adhesive Covering Film
  3. Co-factor 1
  4. Co-factor 2
  5. Co-factor 3
  6. JA1 (10X Wash Buffer)
  7. JA2 (JMJD2 Assay Buffer)
  8. JA3 (JMJD2 Substrate, 50 µg/mL)
  9. JA4 (JMJD2 Assay Standard, 50 µg/mL)
  10. JA5 (Capture Antibody, 1000 µg/mL)
  11. JA6 (Detection Antibody, 400 µg/mL)
  12. JA7 (Developer Solution)
  13. JA8 (Stop Solution)

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for jmjd-2 Activity/Inhibition Assay Kit (Colorimetric)

  • JMJD2

Background

JMJD2 Demethylase Activity/Inhibition Assay Kit (Colorimetric) is use for screening JMJD2 demethylase inhibitors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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