JC-1 mitochondrial membrane potential dependent fluorescent dye

Product Image: JC-1 mitochondrial membrane potential dependent fluorescent dye [NBP2-62754] - Chemical structure of JC-1 (NBP2-62754), a Mitochondrial membrane potential dependent fluorescent dye. Molecular Formula: C25H27Cl4IN4. Molecular Weight: 652.2 g/mol.

• Applications: B/N, NULL

JC-1 mitochondrial membrane potential dependent fluorescent dye Summary

• Description: Mitochondrial membrane potential dependent fluorescent dye
  Purity:>98% (HPLC); NMR (conforms)
  CAS No.: 3520-43-2
  Molecular Formula C₂₅H₂₇Cl₄IN₄
  Molecular Weight:652.2
  Source:Synthetic
  Appearance:Red powder
  PubChem ID: 5492929

Applications/Dilutions

• Dilutions:
  • Adhesion Blockade
  • Product Image
• Application Notes: Red powder. May be dissolved in water (50 mg/mL); DMSO (15 mg/mL).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background

JC-1 is a fluorescent dye that is able to pass across the mitochondrial membrane. It is used to measure mitochondrial membrane potential. At low membrane potentials, it remains a monomer and emits green-fluorescent light (Lambdaex=485 nm, Lambdaem=520 nm). At higher membrane potentials, it forms "J-aggregates", which emit red-fluorescent light (Lambdaex=520-570 nm, Lambdaem=570-610 nm). The ratio of red/green fluorescence is based only on the membrane potential, and not on mitochondrial size, shape or density.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.