Rabbit anti-Human J Chain Secondary Antibody (Azide Free)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide Free
Concentration
LYOPH

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Rabbit anti-Human J Chain Secondary Antibody (Azide Free) Summary

Immunogen
Human J chain is a polypeptide folded within the polymeric structure of the immunoglobulin. J chain isolated form human polymeric IgA and IgM are identical by criteria of composition, peptide maps and antigenicity. Human J chain has been established as distinct from all other component chains of polymeric IgA and IgM. It has a unique primary structure. Antisera to light chains or heavy chains of human Immunoglobulins do not react with J chain. J chain is released from dimeric human myeloma IgA by sulphitolysis and purified by ion exchange chromatography in urea and gel filtration in guanidine. Its purity is tested in alkaline urea polyacrylamide gel electrophoresis, in immunodiffusion and immunoelectrophoresis, showing it to be free of IgA, heavy or light chain contaminants. Freund's complete adjuvant is used in the first step of the immunization procedure.
Clonality
Polyclonal
Host
Rabbit
Purity
Whole Antiserum

Applications/Dilutions

Dilutions
  • Immunoprecipitation 1:10 - 1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antiserum
Preservative
No Preservative
Concentration
LYOPH
Purity
Whole Antiserum
Reconstitution Instructions
Reconstitute the lyophilized antiserum by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Other Available Formats

FITC NBP2-69763
TRITC NBP2-69764

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