| Immunogen | J chain is released from dimeric human myeloma IgA by sulphitolysis and purified by ion exchange chromatography in urea and gel filtration in guanidine. Its purity is tested in alkaline urea polyacrylamide gel electrophoresis, in immunodiffusion and immunoelectrophoresis, showing it to be free of IgA, heavy or light chain contaminants. Freund's complete adjuvant is used in the first step of the immunization procedure. |
| Clonality | Polyclonal |
| Host | Goat |
| Purity | Whole Antiserum |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Whole antiserum |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Whole Antiserum |
| Reconstitution Instructions | Reconstitute the lyophilized antiserum by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). |
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