| Reactivity | HuSpecies Glossary |
| Applications | WB, DB, ELISA, ICC/IF, IHC, WB |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Biotin |
| Concentration | LYOPH |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| FITC | NBP2-69761 | |||
| HRP | NBP2-69762 | |||
| Unconjugated | NBP2-69762 | |||
| Immunogen | Human J chain is a polypeptide folded within the structure of the polymeric immunoglobulin, resulting in a very limited exposure of J chain antigenic determinants. The antiserum is raised against the isolated and purified J chain. J chains isolated from polymeric IgA and IgM are identical by criteria of composition, peptide maps and antigenicity; Human J chain is distinct from all other chain components of polymeric IgA and IgM. It has a unique primary structure as shown by sequence analyses.
Freund's complete adjuvant is used in the first step of the immunization procedure. |
| Specificity | In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), this immunoconjugate shows a single precipitation reaction with totally reduced and alkylated polyclonal and monoclonal polymeric IgA, secretory IgA and IgM. A reaction of complete identity is obtained with precipitated highly purified J chain and with the single of precipitation obtained with normal human serum after two hours incubation with 9M urea and 0.2M mercaptoethanol at pH 8.6. In a competitive radioimmunoassay no inhibition is obtained with monomeric IgA, polyclonal IgG, reduced and alkylated alpha, mu, gamma, kappa and lambda light chains (less than 1 percent over background). |
| Clonality | Polyclonal |
| Host | Goat |
| Purity | Ion exchange chromatography |
| Dilutions |
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| Application Notes | The lyophilized conjugate is shipped at ambient temperature and may be stored at +4C; prolonged storage at or below -20C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. |
| Storage | Store at 4C in the dark. |
| Buffer | Lyophilized from PBS,(pH 7.2) |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Ion exchange chromatography |
| Reconstitution Instructions | Reconstitute by adding 1 ml sterile distilled water, spin down to remove insoluble particles. |
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