![Western Blot: IRIS9 Plus Prestained Protein Ladder [NBP3-33167] - The IRIS9 Plus Prestained Protein Ladder is a combination of 9 pre-stained proteins with molecular weights from 15 to 170 kDa. The 9 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 15 kDa, a red band at 70 kDa and a newly designed peacock green band at 60 kDa serve as reference bands. The IRIS9 Plus Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.](http://images.novusbio.com/fullsize/nbp3-33167_iris9-plus-prestained-protein-ladder-western-blot-23102024121618..png "Western Blot: IRIS9 Plus Prestained Protein Ladder [NBP3-33167] - The IRIS9 Plus Prestained Protein Ladder is a combination of 9 pre-stained proteins with molecular weights from 15 to 170 kDa. The 9 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 15 kDa, a red band at 70 kDa and a newly designed peacock green band at 60 kDa serve as reference bands. The IRIS9 Plus Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.")
| Applications | WB |
| Description | The IRIS9 Plus Prestained Protein Ladder is a combination of 9 pre-stained proteins with molecular weights from 15 to 170 kDa. The 9 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 15 kDa, a red band at 70 kDa and a newly designed peacock green band at 60 kDa serve as reference bands. The IRIS9 Plus Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes. (9 pre-stained bands, 15-170 kDa) |
| Dilutions |
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| Application Notes | 3ul or 5ul per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5ul per well for general Western transferring. |
| Storage | Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 20 mM Trisphosphate at pH 7.5, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol. |
