Iododeoxyuridine/IdU Antibody (32D8.D9) - BSA Free

Western Blot: Iododeoxyuridine/IdU Antibody (32D8.D9) [NBP2-44056] - Western Blot of Iododeoxyuridine/Iododeoxyuridine/IdU antibody (32D8.D9). Lane 1: loading control. Lane 2: FdU. Lane 3: BrdU. Lane 4: Iododeoxyuridine/IdU. Lane 5: CldU. Load: 20 ug per lane.Primary antibody: Anti-Iododeoxyuridine/IdU antibody at 1:1000 for overnight at 4C.Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed: Iododeoxyuridine/IdU. Other band(s): no cross reactive bands were observed for other nucleoside analogs.

N/A: Iododeoxyuridine/IdU Antibody (32D8.D9) [NBP2-44056]

• Reactivity: Hu, All-NA
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 32D8.D9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Iododeoxyuridine/IdU Antibody (32D8.D9) - BSA Free Summary

• Description: This antibody Monoclonal Antibody was purified from ascites fluid by Protein A chromatography
  
  Store this antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Iododeoxyuridine/Iododeoxyuridine/IdU antibody (32D8.D9) was produced in mice by repeated immunizations prepared via immunizations with BromodeoxyUridine-KLH followed by hybridoma development.
• Specificity: This antibody is specific for Iododeoxyuridine/IdU. Cross-reactivity is not observed with BrdU, CldU or FdU.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:10000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Western Blot 1:2000-1:5000
• Application Notes: This product has been tested as suitable for western blot, ELISA, and immunofluorescence microscopy. This antibody may be suitable for additional immunoassays including flow cytometry and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect to detect incorporated IdU thymidine analog from replicated cells.

Reactivity Notes

Cross-reactivity is not observed with BrdU, CldU or FdU.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Alternate Names for Iododeoxyuridine/IdU Antibody (32D8.D9) - BSA Free

• 2'-Deoxy-5-iodouridine
• 54-42-2
• 5-Iodo-2'-deoxyuridine
• 5-Iododeoxyuridine
• Idoxuridin
• Iododeoxyridine
• Iodoxuridine
• Joddeoxiuridin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Iododeoxyuridine/IdU Antibody (NBP2-44056)(5)

Publications using NBP2-44056

• Nair JR, Huang TT, Sunkara A, Pruitt MR et Al. Distinct effects of sacituzumab govitecan and berzosertib on DNA damage response in ovarian cancer iScience 2024-12-04 [PMID: 39628575]
• Gupta N, Huang TT, Nair JR et al. BLM overexpression as a predictive biomarker for CHK1 inhibitor response in PARP inhibitor-resistant BRCA-mutant ovarian cancer Science translational medicine 2023-06-21 [PMID: 37343085]
• Huang TT, Burkett SS, Tandon M et al. Distinct roles of treatment schemes and BRCA2 on the restoration of homologous recombination DNA repair and PARP inhibitor resistance in ovarian cancer Oncogene 2022-10-12 [PMID: 36224341] (ICC/IF, Human)
• A P, Xu X, Wang C et al. EZH2 promotes DNA replication by stabilizing interaction of POLd and PCNA via methylation-mediated PCNA trimerization Epigenetics Chromatin 2018-08-02 [PMID: 30071900] (ICC/IF, Human)
• Betous R, Goullet de Rugy T, Pelegrini AL et al. DNA replication stress triggers rapid DNA replication fork breakage by Artemis and XPF PLoS Genet. 2018-07-01 [PMID: 30059501] (WB, Human)