Iododeoxyuridine/IdU Antibody (32D8.D9)

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Dot Blot: Iododeoxyuridine/IdU Antibody (32D8.D9) [NBP2-44056] - Lane 1: loading control. Lane 2: FdU. Lane 3: BrdU. Lane 4: IdU. Lane 5 CldU. Load: 20 Ug per lane.

Product Details

Summary
Reactivity Hu, All-NASpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
32D8.D9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Iododeoxyuridine/IdU Antibody (32D8.D9) Summary

Immunogen
Anti-IdU monoclonal antibody was produced in mice by repeated immunizations prepared via immunizations with BromodeoxyUridine-KLH followed by hybridoma development.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:5000
  • ELISA 1:1000-1:10000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
Application Notes
Anti-IdU Antibody has been tested as suitable for western blot, ELISA, and immunofluorescence microscopy. This antibody may be suitable for additional immunoassays including flow cytometry and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect to detect incoporated IdU thymidine analog from replicated cells.
Publications
Read Publications using
NBP2-44056 in the following applications:

Reactivity Notes

Cross-reactivity is not observed with BrdU, CldU or FdU. Human reactivity reported in multiple pieces of scientific literature.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Iododeoxyuridine/IdU Antibody (NBP2-44056)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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