Intracellular Staining Flow Cytometry Kit


Flow Cytometry: Intracellular Staining Flow Cytometry Kit [NBP2-29450] - Analysis of intracellular TLR9 in Ramos cells using IC-Flow kit to fix and permeabilize cells. Blue: Unstained Ramos cells, Green: Isotype more

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Intracellular Staining Flow Cytometry Kit Summary

This kit is optimized for intracellular staining of cells in flow cytometric applications. It is designed and optimized to minimize non-specific staining while maximizing signal-to-noise ratio for clear and consistent data.
Kit Type
Flow Cytometry Kit


  • Flow (Intracellular)
  • Flow Cytometry
Application Notes
Brefeldin A is an inhibitor of intracellular protein transport. It blocks translocation of proteins from the endoplasmic reticulum to the Golgi apparatus and can enhance detection of some intracellular proteins. Researchers are encouraged to determine whether or not Brefeldin A is needed to assay for the protein of interest. Brefeldin A is light-sensitive, store at 4C in the dark. It should be diluted in culture media (1:1000) just prior to use. Add to cell culture and incubate for 4hr before harvesting cells. Use in Flow Intracellular reported in scientific literature (PMID 24804954).
Read Publications using
NBP2-29450 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24804954).

Packaging, Storage & Formulations

Storage of components varies. See protocol for specific instructions.

Kit Components

  1. 1000X Brefeldin A (100 uL)
  2. 10X Permeabilization Buffer (2 x 60 mL)
  3. 1X Fixation Buffer (60 mL)
  4. 1X Staining Buffer (2 x 60 mL)

Alternate Names for Intracellular Staining Flow Cytometry Kit

  • Permeabilization buffer


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
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Publications for Intracellular Staining Kit (NBP2-29450)(6)

We have publications tested in 2 confirmed species: Human, Canine.

We have publications tested in 5 applications: FLOW, Flow, Flow-IC, ICC/IF, WB.

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Showing Publications 1 - 6 of 6.
Publications using NBP2-29450 Applications Species
Zechner C, Henne WM, Sathe AA et al. Cellular abundance of sodium phosphate co-transporter SLC20A1/PiT1 and phosphate uptake are controlled post-transcriptionally by ESCRT The Journal of biological chemistry Apr 18 2022 [PMID: 35447110] (Flow) Flow
Valencia Pacheco Guillermo J, Pinzon Herrera Francisc, Cruz Lopez Juan J et al. Expression and activation of intracellular receptors TLR7, TLR8 and TLR9 in peripheral blood monocytes from HIV-infected patients. Colomb Med (Cali). 2013 Apr [PMID: 24892454] (FLOW) FLOW
Pradhan N, Pratheek BM, Garai A et al. Induction of apoptosis by Fe(salen)Cl through caspase-dependent pathway specifically in tumor cells. Cell Biol. Int. 2014 May 07 [PMID: 24804954] (Flow-IC, Human)

Jurkat, Figs 8,9.
Flow-IC Human
Sathe A, Reddy KV. TLR9 and RIG-I signaling in human endocervical epithelial cells modulates inflammatory responses of macrophages and dendritic cells in vitro. PLoS ONE. 2014 Jan 10 [PMID: 24409285] (WB, Flow-IC, ICC/IF, Human)

End1/E6E7 endocervical epithelial cells: Flow (Intracellular), Fig 1C; IF (confocal microscopy), Fig 1D; WB, Figs S1A, S1C. Both CpG-ODN and Poly(I:C) stimulation upregulated TLR9 expression. TLR9 was detected at ~100 kDa in WB. Mouse monoclonal against T
WB, Flow-IC, ICC/IF Human
Chamberlain ND, Kim SJ, Vila OM et al. Ligation of TLR7 by rheumatoid arthritis synovial fluid single strand RNA induces transcription of TNF-alpha in monocytes. Ann Rheum Dis. 2013 Mar [PMID: 22730373]
Burgener IA, Jungi TW. Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol. 2008 Jul 15 [PMID: 18439687] (Flow-IC, Canine)

Canine (dog) PBMC subpopulations: 1. 10083K [IC-Flow (Intracellular Staining Flow Assay) Kit.
Flow-IC Canine

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Product General Protocols

View specific protocols for Intracellular Staining Kit (NBP2-29450): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Intracellular Staining Kit (NBP2-29450). (Showing 1 - 1 of 1 FAQs).

  1. Does your Intracellular Toll-like Receptor Staining Flow Assay Kit [NBP2-29450] contain Sodium Azide (NAN3)? If yes, how much NAN3 does it have?
    • Yes, there is Sodium Azide in the permeablization and staining buffers in this kit. Permeablization and staining buffers contain 0.05 % sodium azide. Use caution when handling. All the materials included in this kit should be treated as hazardous materials and be disposed of accordingly.

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