Human Insulin Autoantibodies ELISA Kit (Colorimetric) Summary
This assay has high sensitivity and excellent specificity for detection of human IAA. No significant cross-reactivity or interference between human IAA and analogues was observed.
|Standard Curve Range
%CV < 20
%CV < 15
50 - 100 uL
ELISA Kit (Colorimetric)
Packaging, Storage & Formulations
- Stop Solution
- Sample Diluent
- Substrate A
- Substrate B
- Wash Buffer (20 x concentrate)
- Adhesive Strip (For 96 wells)
- Coated assay plate
- Instruction manual
- Positive Control
- Negative Control
Please contact our Technical Services with any questions regarding species reactivity
This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with purified antigen. Samples are pipetted into the wells with anti-human IgG conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human IAA bound in the initial step. The color development is stopped and the intensity of the color is measured.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed
for 6 months from date of receipt.
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