Human Insulin Autoantibodies ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
serum, plasma
Standard Curve Range
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Order Details

Human Insulin Autoantibodies ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of human IAA. No significant cross-reactivity or interference between human IAA and analogues was observed.
Standard Curve Range
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Assay Type
Indirect ELISA
Inter-Assay
%CV < 20
Intra-Assay
%CV < 15
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Stop Solution
  2. Sample Diluent
  3. Substrate A
  4. Substrate B
  5. Wash Buffer (20 x concentrate)
  6. Adhesive Strip (For 96 wells)
  7. Coated assay plate
  8. Instruction manual
  9. HRP-conjugate
  10. Positive Control
  11. Negative Control

Notes

Please contact our Technical Services with any questions regarding species reactivity

Background

This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with purified antigen. Samples are pipetted into the wells with anti-human IgG conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human IAA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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