INSTA-Blot Rat Tissues Summary
Ready-to-use PVDF membrane with 11 rat tissue lysates (20 ug total protein per lane)
INSTA-Blot™is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot™ Rat Tissues NBP2-30112 is a simple and fast solution for screening proteins from various rat tissues. We've streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS-PAGE and electro-blotting steps. With INSTA-Blot™, imm...unoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop.
INSTA-Blot™ Rat Tissues NBP2-30112 contains denatured proteins from rat lysates loaded at 20 ug (total protein, Bradford Assay) per lane on a 4-20% Tris-Glycine mini gel. After resolution by
SDS-PAGE at 125V for 1 hour the proteins are electro-blotted for 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. The INSTA-Blot™ is sold ready-to-use in your western blot protocol.
Tissues are from Sprague Dawley rats. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol).
Packaging, Storage & Formulations
Store at room temperature.
The molecular weights for the marker used with this product are: 180, 116, 97, 66, 44, 29, 18, 14, 6.
Alternate Names for INSTA-Blot Rat Tissues
- INSTA Blot Rat Tissues
- INSTABlot Rat Tissues
- INSTA-Blot Rat
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Western Blot Membranes are guaranteed
for 1 year from date of receipt.
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