INSTA-Blot Rat Tissues

SDS-Page: INSTA-Blot Rat Tissues [NBP2-30112] - Rat Tissues. Approximately 20 ug per lane of different rat tissue lysate are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker; 2. Brain; 3. Heart; 4. Small Intestine; 5. Kidney; 6. Liver; 7. Lung; 8. Skeletal Muscle; 9. Stomach; 10. Spleen; 11. Ovary; 12. Testis

INSTA-Blot Rat Tissues Summary

• Description: Ready-to-use PVDF membrane with 11 rat tissue lysates (20 ug total protein per lane)
• Background: INSTA-Blot™is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot™ Rat Tissues NBP2-30112 is a simple and fast solution for screening proteins from various rat tissues. We've streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS-PAGE and electro-blotting steps. With INSTA-Blot™, imm...unoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop.
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• Preparation Method: INSTA-Blot™ Rat Tissues NBP2-30112 contains denatured proteins from rat lysates loaded at 20 ug (total protein, Bradford Assay) per lane on a 4-20% Tris-Glycine mini gel. After resolution by SDS-PAGE at 125V for 1 hour the proteins are electro-blotted for 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. The INSTA-Blot™ is sold ready-to-use in your western blot protocol.

Applications/Dilutions

• Application Notes: Tissues are from Sprague Dawley rats. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol).

Packaging, Storage & Formulations

• Storage: Store at room temperature.

Notes

The molecular weights for the marker used with this product are: 180, 116, 97, 66, 44, 29, 18, 14, 6.

Alternate Names for INSTA-Blot Rat Tissues

• INSTA Blot Rat Tissues
• INSTABlot Rat Tissues
• INSTA-Blot Rat

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Western Blot Membranes are guaranteed for 1 year from date of receipt.

Publications for INSTA-Blot Rat Tissues (NBP2-30112)(2)

Publications using NBP2-30112

• Bach JP, Borta H, Ackermann W et al. The secretory granule protein syncollin localizes to HL-60 cells and neutrophils. J Histochem Cytochem. 2006-08-01 [PMID: 16517980]
• Berleth ES, Masso-Welch PA, Kazim LA et al. Expression, tissue distribution, and cellular localization of the antiapoptotic TIP-B1 protein. J Leukoc Biol. 2001-06-01 [PMID: 11404387]