INSTA-Blot Rat Brain

SDS-Page: INSTA-Blot Rat Brain [NBP2-30121] - Approximately 30 ug per lane of tissue lysate is resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker; 2-12 rat brain.

INSTA-Blot Rat Brain Summary

• Description: Ready-to-use PVDF membrane with rat brain lysate (30 ug total protein per lane).
• Background: INSTA-Blot™ is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot™ Rat Brain NBP2-30121 is a simple and fast solution for screening proteins from various human, mouse and rat tissues. We've streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS-PAGE and electro-blotting steps. With INST...A-Blot™, immunoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop.
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• Preparation Method: The INSTA-Blot™ Rat Brain NBP2-30121 contains denatured proteins from tissue lysates loaded at 30 ug (total protein, Bradford Assay) per lane on a 4-20% Tris-Glycine mini gel. After resolution by SDS-PAGE at 125V for 1 hour the proteins are electro-blotted for 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. The INSTA-Blot™ is provided ready-to-use in your western blot protocol.

Applications/Dilutions

• Application Notes: Rat tissues are from Sprague Dawley rats. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol).

Packaging, Storage & Formulations

• Storage: Store at room temperature.

Alternate Names for INSTA-Blot Rat Brain

• INSTA Blot Rat Brain
• INSTABlot Rat Brain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Western Blot Membranes are guaranteed for 1 year from date of receipt.

Publications for INSTA-Blot Rat Brain (NBP2-30121)(1)

Publication using NBP2-30121

• Privette Vinnedge LM, Benight NM, Wagh PK et al. The DEK oncogene promotes cellular proliferation through paracrine Wnt signaling in Ron receptor-positive breast cancers. Oncogene. 2014-06-23 [PMID: 24954505] (IHC-P, Human)
  
  Details:
  Figs 6D, S5A