INSTA-Blot Multi-species Stomach, Small Intestine and Pancreas

SDS-Page: INSTA-Blot Multi-species Stomach, Small Intestine and Pancreas [NBP2-30118] - Approximately 20 ug per lane of tissue lysates and resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker; 2. human stomach; 3. mouse stomach; 4. rat stomach; 5. Molecular weight marker; 6. human small intestine; 7. mouse small intestine; 8. rat small intestine; 9. Molecular weight marker; 10. human pancreas; 11. mouse pancreas; 12. rat pancreas.

INSTA-Blot Multi-species Stomach, Small Intestine and Pancreas Summary

• Description: Ready-to-use PVDF membrane with 11 human, mouse and rat tissue lysates (20 ug total protein per lane).
• Background: INSTA-Blot™ is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot™ Multi-species Stomach, Small Intestine, Pancreas Tissues NBP2-30118 is a simple and fast solution for screening proteins from various human, mouse and rat tissues. We've streamlined the western blotting procedure by eliminating the sample aquisition, preparation,... SDS-PAGE and electro-blotting steps. With INSTABlot™, immunoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop.
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• Preparation Method: The INSTA-Blot™ Multi-species Stomach, Small Intestine, Pancreas Tissues NBP2-30118 contains denatured proteins from tissue lysates loaded at 20 ug (total protein, Bradford Assay) per lane on a 4 20% Tris-Glycine mini gel. After resolution by SDSPAGE at 125V for 1 hour the proteins are electro-blotted for 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. The INSTA-Blot™ is provided ready-touse in your western blot protocol.

Applications/Dilutions

• Application Notes: Human tissues are from donors with no known disease. Mouse tissues are from Balb/c mice. Rat tissues are from Sprague Dawley rats. Tissues are lysed in lysis buffer (10 mM Tris, pH = 8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol).

Packaging, Storage & Formulations

• Storage: Store at room temperature.

Alternate Names for INSTA-Blot Multi-species Stomach, Small Intestine and Pancreas

• INSTA Blot Multi-species Stomach, Small Intestine, Pancreas
• INSTA-Blot Multi-species Stomach, Small Intestine and Pancreas
• INSTA-Blot Multi-species Stomach, Small Intestine, Pancreas
• INSTABlot Multi-species Stomach, Small Intestine, Pancreas

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Western Blot Membranes are guaranteed for 1 year from date of receipt.

Publications for INSTA-Blot Multi-species Stomach, Small Intestine and Pancreas (NBP2-30118)(1)

Publication using NBP2-30118

• Zhou M, Xia L, Wang J. Metformin transport by a newly cloned proton-stimulated organic cation transporter (plasma membrane monoamine transporter) expressed in human intestine. Drug Metab Dispos. 2007-10-01 [PMID: 17600084] (WB, Human)
  
  Details:
  Products cited for WB (human small intestine, Fig 6B): 1. GAPDH (IMG-5019A-1, -2) 2. INSTA-BLOT containing human small intenstine tissue lysate (IMB-103, IMB-123).