SDS-Page: INSTA-Blot Multi-species Stomach, Small Intestine and Pancreas [NBP2-30118] - Approximately 20 ug per lane of tissue lysates and resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido ...read more
INSTA-Blot Multi-species Stomach, Small Intestine and Pancreas Summary
Ready-to-use PVDF membrane with 11 human, mouse and rat tissue lysates (20 ug total protein per lane).
INSTA-Blot™ is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot™ Multi-species Stomach, Small Intestine, Pancreas Tissues NBP2-30118 is a simple and fast solution for screening proteins from various human, mouse and rat tissues. We've streamlined the western blotting procedure by eliminating the sample aquisition, preparation,... SDS-PAGE and electro-blotting steps. With INSTABlot™, immunoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop.
The INSTA-Blot™ Multi-species Stomach, Small Intestine, Pancreas Tissues NBP2-30118 contains denatured proteins from tissue lysates loaded at 20 ug (total protein, Bradford Assay) per lane on a 4 20% Tris-Glycine mini gel. After resolution by SDSPAGE at 125V for 1 hour the proteins are electro-blotted for 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. The INSTA-Blot™ is provided ready-touse in your western blot protocol.
Human tissues are from donors with no known disease. Mouse tissues are from Balb/c mice. Rat tissues are from Sprague Dawley rats. Tissues are lysed in lysis buffer (10 mM Tris, pH = 8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol).
Read Publication using NBP2-30118 in the following applications: