INSTA-Blot Multi-species Brain,Testis and Ovary

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INSTA-Blot Multi-species Brain,Testis and Ovary [NBP2-30115] - Approximately 20 ug per lane of tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular ...read more

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Summary
Reactivity HuSpecies Glossary

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INSTA-Blot Multi-species Brain,Testis and Ovary Summary

Description
Ready-to-use PVDF membrane with 11 human, mouse and rat tissue lysates (20 ug total protein per lane).
Background
INSTA-Blot™ is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot™ Multi-species Brain, Testis and Ovary NBP2-30115 is a simple and fast solution for screening proteins from various human, mouse and rat tissues. We've streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDSPAGE and electr...o-blotting steps. With INSTA-Blot™, immunoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop.
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Preparation
Method
The INSTA-Blot™ Multi-species Brain, Testis and Ovary NBP2-30115 contains denatured proteins from tissue lysates loaded at 20 ug (total protein, Bradford Assay) per lane on a 4-20% Tris-Glycine mini gel. After resolution by SDS-PAGE at 125V for 1 hour the proteins are electro-blotted for 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. The INSTA-Blot™ is provided ready-to-use in your western blot protocol.

Applications/Dilutions

Application Notes
Human tissues are from donors with no known disease. Mouse tissues are from Balb/c mice. Rat tissues are from Sprague Dawley rats. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol).
Publications
Read Publication using NBP2-30115.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 23243155)

Packaging, Storage & Formulations

Storage
Store at room temperature.

Notes

Mark12 is used as the molecular weight marker for this product.

Alternate Names for INSTA-Blot Multi-species Brain,Testis and Ovary

  • INSTA Blot Multi-species Brain,Testis and Ovary
  • INSTA-Blot Multi-species Brain, Testis&Ovary
  • INSTABlot Multi-species Brain,Testis and Ovary

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Western Blot Membranes are guaranteed for 1 year from date of receipt.
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Publications for INSTA-Blot Multi-species Brain,Testis and Ovary (NBP2-30115)(1)

We have publications tested in 1 confirmed species: Human.


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Publication using NBP2-30115 Applications Species
Flaumenhaft Robert. alpha-granules: a story in the making. Blood. 2012 Dec 13 [PMID: 23243155] (Human) Human

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Other Available Formats

NBP2-30115

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