INSTA-Blot Male Lung Tissue OncoPair

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Western Blot: INSTA-Blot Male Lung Tissue OncoPair [NBP2-30122] - Representative amido-black stained blot. Samples on the blot are laid out in pairs of tumor and normal tissue lysate from the same patient. There are ...read more
INSTA-Blot Male Lung Tissue OncoPair [NBP2-30122] - Tumor tissue is taken from healthy tumor tissue while normal tissue is taken from an area adjacent to the tumor tissue.

Product Details

Summary
Applications WB

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INSTA-Blot Male Lung Tissue OncoPair Summary

Description
Tissue
Lane Tissue Type Cat. No. Grade Stage Sex Age Diagnosis
MWM
2 Lung NBP2-27721 2 I M 45 Squamous cell carcinoma
3 Lung NBP2-27719 Normal adjacent
4 Lung NBP2-27726 2 I M 51 Squamous cell carcinoma
5 Lung NBP2-27724 Normal adjacent
6 Lung NBP2-27731 2 I M 74 Squamous cell carcinoma
7 Lung NBP2-27729 Normal adjacent
8 Lung NBP2-27765 2 I M 58 Squamous cell carcinoma
9 Lung NBP2-27763 Normal adjacent
10 Lung NBP2-27775 2 I M 69 Squamous cell carcinoma
11 Lung NBP2-27773 Normal adjacent
12 Lung NBP2-27780. 2 I M 70 Squamous cell carcinoma
13 Lung NBP2-27778 Normal adjacent
14 Lung NBP2-27785 2 I M 50 Squamous cell carcinoma
15 Lung NBP2-27783 Normal adjacent
Background
The development of biomarkers has enormous potential for revolutionizing the diagnosis and treatment of disease. Western blot analysis is an integral technique for biomarker profiling. While key for biomarker discovery, the existing body of western blot data has primarily been defined from studies of tumor, immortal, and primary cells growing in vitro. Collectively, results obtained over dec...ades have been integral to the dogma that up- and down-regulation of proteins can be leveraged as biomarkers of normal development, homeostasis, and disease. However, data from human tissue-derived products is key for generating biomarker validation data and progressing the most promising biomarker targets towards the clinic. The OncoPair INSTA-Blot͐ product line addresses the increasing demand for the inclusion of human tissue-derived products in biomarker profiling.

The OncoPair INSTA-Blot͐ is a ready-to-use PVDF western blot membrane containing denatured protein lysates manufactured from Novus extensive Human Clinical Tissue Lysate collection. Each blot contains 14 lanes of alternating tumor (T) and matched normal adjacent (NA) tissue lysates from 7 patient donors. Incorporating both T and NA on the blot enables protein expression analysis of the tumor and microenvironment. The tumor microenvironment is increasingly being recognized as a major factor in influencing malignant progression and metastatic potential.

Cancer is a heterogeneous disease and the presence of multiple donors on a single blot enables rapid screening of protein expression variability between T/NA from different individuals and at different disease stages. OncoPair INSTA-Blots͐ are available for a wide range of tumor types and stages. The product line is manufactured from donor patient tissue obtained from established hospital-based collection sites around the world. Tissues are collected under strict bioethical standards using IRB and HIPAA approved protocols. These protocols ensure patient confidentiality, safety, and informed consent. Tissues are flash frozen at collection sites within minutes of removal, maintained in liquid nitrogen and processed into lysates using protocols optimized for extracting proteins from tissues. Lysates are denatured for OncoPair INSTA-Blot͐ production; native lysates are available for follow up proteomic studies. OncoPair INSTA-Blots͐ and lysates are also useful in conjunction with the Cell Line and Normal Tissue INSTA-Blots͐ and lysates for comprehensive antibody validation and to profile antibody reactivity and protein expression patterns across species and between tissues and cells lines. The OncoPair INSTA-Blots͐ are proteomic discovery tools and are useful for screening the expression of various proteins in tumor and normal adjacent tissues from various donors. Researchers may want to follow up on their results by further analysis with the corresponding individual lysates when available.
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Preparation
Method
Tissue specimens were homogenized in modified RIPA buffer to obtain the soluble proteins and centrifuged to clarify. The concentration of protein in each lysate was determined by a standard protein assay and standardized to 1 mg/ml. Sample buffer was added to the soluble fraction and approximately 14 ug/lane of protein was run and then transferred to PVDF membrane. Soluble fraction extraction buffer (extract 1): PBS at pH 7.4, 1 ug/ml Aprotinin, 1 mM NaF -Modified RIPA buffer: 1 mM EDTA, 1 ug/ml Pepstatin-A, 0.1% SDS, 0.25% Na deoxycholate, 1 ug/ml Leupeptin, 1 mM PMSF, 1 mM Na3VO4 5X Sample buffer: 50 ml glycerol, 15 g SDS, 3.819 g Tris, pH 6.8, 25 ml 2-ME, 100 mg bromophenol blue, final volume of 100 ml with DI H2O.

Applications/Dilutions

Application Notes
Use in Western blot reported in scientific literature (PMID 25985210)
Publications
Read Publications using
NBP2-30122 in the following applications:

  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at room temperature.

Notes

The molecular weights for the marker used with this product are: 180, 116, 97, 66, 44, 29, 18, 14, 6.

Alternate Names for INSTA-Blot Male Lung Tissue OncoPair

  • INSTA Blot Male Lung Tissue OncoPair
  • INSTABlot Male Lung Tissue OncoPair
  • INSTA-Blot Male Lung Tissue

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Western Blot Membranes are guaranteed for 1 year from date of receipt.
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Publications for INSTA-Blot Male Lung Tissue OncoPair (NBP2-30122)(5)

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Product General Protocols

View specific protocols for INSTA-Blot Male Lung Tissue OncoPair (NBP2-30122): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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WB Video Protocol

FAQs for INSTA-Blot Male Lung Tissue OncoPair (NBP2-30122). (Showing 1 - 1 of 1 FAQs).

  1. Is the membrane with the catalog number NBP2-30122 same as NBP2-30125?ndFragment-->
    • No, they are not same. NBP2-30122 is INSTA-Blot Male Lung Tissue OncoPair, which corresponds to Imgenex product number IMB-127a (Imgenex is part of Novus).http://www.imgenex.com/imb_details.php?catalog_no=IMB-127aNBP2-30125 is INSTA-Blot Breast Tissue OncoPair, which corresponds to Imgenex product number IMB-130bhttp://www.imgenex.com/imb_details.php?catalog_no=IMB-130b

Other Available Formats

NBP2-30122

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