INSTA-Blot Human Tissues


SDS-Page: INSTA-Blot Human Tissues [NBP2-31378] - Approximately 50 ug per lane of human tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight more

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Applications WB
Concentration is not relevant for this product. Please see the protocols for proper use of this product.

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INSTA-Blot Human Tissues Summary

Ready-to-use PVDF membrane with 11 human tissue lysates (50 ug total protein per lane).
INSTA-Blot(R) is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot(R); Human Tissues NBP2-31378 is a simple and fast solution for screening proteins from various human tissues. We've streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS-PAGE and electro-blotting steps. With INSTA-Blot(R);, immuno...blotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop.
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INSTA-Blot͐ Human Tissues. NBP2-31378 contains denatured proteins from human lysates loaded at 50 ug (total protein, Bradford Assay) per lane on a 4-20% Tris-Glycine mini gel. After resolution by SDS-PAGE at 125V for 1 hour the proteins are electro-blotted for 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. The INSTA-Blot͐ is sold ready-to-use in your western blot protocol.

Packaging, Storage & Formulations

Store at room temperature.
Concentration is not relevant for this product. Please see the protocols for proper use of this product.


Application Notes
Tissues are from donors with no known disease. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol). Use in Western blot reported in scientific literature (PMID 25615412)
Read Publications using NBP2-31378.


The molecular weights for the marker used with this product are: 180, 116, 97, 66, 44, 29, 18, 14, 6.

Alternate Names for INSTA-Blot Human Tissues

  • INSTA Blot Human Tissues
  • INSTABlot Human Tissues
  • INSTA-Blot Human


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Western Blot Membranes are guaranteed for 1 year from date of receipt.
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Publications for INSTA-Blot Human Tissues (NBP2-31378)(2)

We have publications tested in 1 application: WB.

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Product General Protocols

View specific protocols for INSTA-Blot Human Tissues (NBP2-31378): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for INSTA-Blot Human Tissues (NBP2-31378). (Showing 1 - 1 of 1 FAQs).

  1. I need to use the pancreas tissue as a positive control with my western blot in 12% acrylamide gel. Is it possible to have NBP2-30113 in high gel percentage 12% acrylamide?
    • We manufacture the blots using 4-20% gradient gels as denoted on the data sheet link. All of the lysates we use to generate the blot are available separately. By purchasing individual lysates you can run them on the gel type of your choice.

Additional INSTA-Blot Human Tissues Products

INSTA-Blot Human Tissues NBP2-31378

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