SDS-Page: INSTA-Blot Human Tissues [NBP2-31378] - Approximately 50 ug per lane of human tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight ...read more
Ready-to-use PVDF membrane with 11 human tissue lysates (50 ug total protein per lane).
INSTA-Blot(R) is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot(R); Human Tissues NBP2-31378 is a simple and fast solution for screening proteins from various human tissues. We've streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS-PAGE and electro-blotting steps. With INSTA-Blot(R);, immuno...blotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop.
INSTA-Blot͐ Human Tissues. NBP2-31378 contains denatured proteins from human lysates loaded at 50 ug (total protein, Bradford Assay) per lane on a 4-20% Tris-Glycine mini gel. After resolution by SDS-PAGE at 125V for 1 hour the proteins are electro-blotted for 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. The INSTA-Blot͐ is sold ready-to-use in your western blot protocol.
Tissues are from donors with no known disease. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol). Use in Western blot reported in scientific literature (PMID 25615412)
FAQs for INSTA-Blot Human Tissues (NBP2-31378). (Showing 1 - 1 of 1 FAQs).
I need to use the pancreas tissue as a positive control with my western blot in 12% acrylamide gel. Is it possible to have NBP2-30113 in high gel percentage 12% acrylamide?
We manufacture the blots using 4-20% gradient gels as denoted on the data sheet link. All of the lysates we use to generate the blot are available separately. By purchasing individual lysates you can run them on the gel type of your choice.