INSTA-Blot Human Tissues

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Western Blot: INSTA-Blot Human Tissues [NBP2-30113] - Human Tissues. Approximately 20 ug per lane of human tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

INSTA-Blot Human Tissues Summary

Description
Ready-to-use PVDF membrane with 11 human tissue lysates (20 ug total protein per lane).
Background
INSTA-Blot͐ is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot͐ Human Tissues NBP2-30113 is a simple and fast solution for screening proteins from various human tissues. We've streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS-PAGE and electro-blotting steps. With INSTA-Blot͐,... immunoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop.
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Preparation
Method
INSTA-Blot͐ Human Tissues NBP2-30113 contains denatured proteins from human lysates loaded at 20 ug (total protein, Bradford Assay) per lane on a 4-20% Tris-Glycine mini gel. After resolution by SDS-PAGE at 125V for 1 hour the proteins are electro-blotted for 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. The INSTA-Blot͐ is sold ready-to-use in your western blot protocol

Applications/Dilutions

Application Notes
Tissues are from donors with no known disease. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol). Use in western blot reported in scientific literature (PMID: 24704276).
Publications
Read Publications using
NBP2-30113 in the following applications:

  • IHC
    1 publication
  • WB
    7 publications

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24704276)

Packaging, Storage & Formulations

Storage
Store at room temperature.

Notes

Mark12 is used as the molecular weight marker for this product.

Alternate Names for INSTA-Blot Human Tissues

  • INSTA Blot Human Tissues
  • INSTABlot Human Tissues
  • INSTA-Blot Human

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Western Blot Membranes are guaranteed for 1 year from date of receipt.
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Publications for INSTA-Blot Human Tissues (NBP2-30113)(16)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC, WB.


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IHC
(1)
WB
(7)
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Human
(5)
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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NBP2-30113 Applications Species
Samyesudhas SJ, Jroy L, Cowden Dahl KD. Differential expression of ARID3B in normal adult tissue and carcinomas. Gene 2014 Jun 10 [PMID: 24704276] (WB, Human)

Details:
Fig 3: The NBP2-30113 blot was stained with ARID3B-FL and ARID3B-Sh antibodies. Ponceau S staining was used as a loading control.
WB Human
Zuleger Nikolaj, Boyle Shelagh, Kelly David A et al. Specific nuclear envelope transmembrane proteins can promote the location of chromosomes to and from the nuclear periphery. Genome Biol. 2013 [PMID: 23414781] (WB, Human) WB Human
Yoshikawa Noritada, Shimizu Noriaki, Maruyama Takako et al. Cardiomyocyte-specific overexpression of HEXIM1 prevents right ventricular hypertrophy in hypoxia-induced pulmonary hypertension in mice. PLoS One. 2012 [PMID: 23300697] (IHC) IHC
Uhrigshardt H, Singh A, Kovtunovych G et al. Characterization of the human HSC20, an unusual DnaJ type III protein, involved in iron-sulfur cluster biogenesis. Hum Mol Genet. 2010 Oct 1 [PMID: 20668094]
Zhou You, Li Shiqian, Mayranpaa Mikko I et al. OSBP-related protein 11 (ORP11) dimerizes with ORP9 and localizes at the Golgi-late endosome interface. Exp Cell Res. 2010 Nov 15 [PMID: 20599956] (WB, Human) WB Human
Romanish Mark T, Nakamura Hisae, Lai C Benjamin et al. A novel protein isoform of the multicopy human NAIP gene derives from intragenic Alu SINE promoters. PLoS One. 2009 [PMID: 19488400] (WB) WB
Bernstein HG, Stricker R, Dobrowolny H et al. Histochemical evidence for wide expression of the metalloendopeptidase nardilysin in human brain neurons. Neuroscience. 2007 Jun 8 [PMID: 17442499]
Hayashi R, Goto Y, Ikeda R et al. CDCA4 is an E2F transcription factor family-induced nuclear factor that regulates E2F-dependent transcriptional activation and cell proliferation. J Biol Chem. 2006 Nov 24 [PMID: 16984923]
Nunez Maria I, Ludes-Meyers John, Aldaz C Marcelo. WWOX protein expression in normal human tissues. J Mol Histol. 2006 May [PMID: 16941225] (WB, Human) WB Human
Paulsen Michelle T, Starks Adrienne M, Derheimer Frederick A et al. The p53-targeting human phosphatase hCdc14A interacts with the Cdk1/cyclin B complex and is differentially expressed in human cancers. Mol Cancer. 2006 [PMID: 16784539] (WB, Human) WB Human
Show All 16 Publications.

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Product General Protocols

View specific protocols for INSTA-Blot Human Tissues (NBP2-30113): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for INSTA-Blot Human Tissues (NBP2-30113). (Showing 1 - 1 of 1 FAQs).

  1. I need to use the pancreas tissue as a positive control with my western blot in 12% acrylamide gel. Is it possible to have NBP2-30113 in high gel percentage 12% acrylamide?
    • We manufacture the blots using 4-20% gradient gels as denoted on the data sheet link. All of the lysates we use to generate the blot are available separately. By purchasing individual lysates you can run them on the gel type of your choice.

Other Available Formats

NBP2-30113

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