INSTA-Blot Colon Tissue OncoPair

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SDS-Page: INSTA-Blot Colon Tissue OncoPair [NBP2-30129] - Samples on the blot are layed out in pairs of tumor and normal tissue lysate from the same patient. There are seven pairs of samples, representing seven ...read more
INSTA-Blot Colon Tissue OncoPair [NBP2-30129] - Tumor tissue is taken from healthy tumor tissue while normal tissue is taken from an area adjacent to the tumor tissue.

Product Details

Summary
Applications WB

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INSTA-Blot Colon Tissue OncoPair Summary

Description
Tissue
Lane Tissue Type Cat. No. Grade Stage Sex Age Diagnosis
MWM
2 Colon NBP2-28142 1 I M 75 Adenocarcinoma
3 Colon NBP2-28140 Normal adjacent
4 Colon NBP2-28146 1 I M 25 Adenocarcinoma
5 Colon NBP2-28144 Normal adjacent
6 Colon NBP2-28184 1 II M 77 Adenocarcinoma
7 Colon NBP2-28182 Normal adjacent
8 Colon NBP2-28263 1 II F 38 Adenocarcinoma
9 Colon NBP2-28261 Normal adjacent
10 Colon NBP2-28273 1 II F 46 Adenocarcinoma
11 Colon NBP2-28271 Normal adjacent
12 Colon NBP2-28306. 1 IIIA F 66 Adenocarcinoma
13 Colon NBP2-28304 Normal adjacent
14 Colon NBP2-28311 1 IIIA F 43 Adenocarcinoma
15 Colon NBP2-28309 Normal adjacent
Background
The development of biomarkers has enormous potential for revolutionizing the diagnosis and treatment of disease. Western blot analysis is an integral technique for biomarker profiling. While key for biomarker discovery, the existing body of western blot data has primarily been defined from studies of tumor, immortal, and primary cells growing in vitro. Collectively, results obtained over de...cades have been integral to the dogma that up- and down-regulation of proteins can be leveraged as biomarkers of normal development, homeostasis, and disease. However, data from human tissue-derived products is key for generating biomarker validation data and progressing the most promising biomarker targets towards the clinic. The OncoPair INSTA-Blot™ product line addresses the increasing demand for the inclusion of human tissue-derived products in biomarker profiling. The OncoPair INSTA-Blot™ is a ready-to-use PVDF western blot membrane containing denatured protein lysates manufactured from Novus's extensive Human Clinical Tissue Lysate collection. Each blot contains 14 lanes of alternating tumor (T) and matched normal adjacent (NA) tissue lysates from 7 patient donors. Incorporating both T and NA on the blot enables protein expression analysis of the tumor and microenvironment. The tumor microenvironment is increasingly being recognized as a major factor in influencing malignant progression and metastatic potential. Cancer is a heterogeneous disease and the presence of multiple donors on a single blot enables rapid screening of protein expression variability between T/NA from different individuals and at different disease stages. Clinical diagnosis and histopathology reports from board-certified pathologists are available for every patient sample. OncoPair INSTA-Blots™ are available for a wide range of tumor types and stages. The product line is manufactured from donor patient tissue obtained from established hospital-based collection sites around the world. Tissues are collected under strict bioethical standards using IRB and HIPAA approved protocols. These protocols ensure patient confidentiality, safety, and informed consent. Tissues are flash frozen at collection sites within minutes of removal, maintained in liquid nitrogen and processed into lysates using protocols optimized for extracting proteins from tissues. Lysates are denatured for OncoPair INSTA-Blot™ production; native lysates are available for follow up proteomic studies. OncoPair INSTA-Blots™ and lysates are also useful in conjunction with the Cell Line and Normal Tissue INSTA-Blots™ and lysates for comprehensive antibody validation and to profile antibody reactivity and protein expression patterns across species and between tissues and cells lines.
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Preparation
Method
Tissue specimens were homogenized in modified RIPA buffer to obtain the soluble proteins and centrifuged to clarify. Sample buffer was added to the soluble fraction and approximately 14 ug/lane of protein was run and then transfered to PVDF membrane. -Soluble fraction extraction buffer (extract 1): PBS at pH 7.4, 1 ug/ml Aprotinin, 1 mM NaF -Modified RIPA buffer: 1 mM EDTA, 1 ug/ml Pepstatin-A, 0.1% SDS, 0.25% Na deoxycholate, 1 ug/ml Leupeptin, 1 mM PMSF, 1 mM Na3VO4 -5X Sample buffer: 50 ml glycerol, 15 g SDS, 3.819 g Tris, pH 6.8, 25 ml 2-ME, 100 mg bromophenol blue, final volume of 100 ml with DI H2O

Applications/Dilutions

Application Notes
Use in Western blot reported in scientific literature (PMID 25985210)
Publications
Read Publications using
NBP2-30129 in the following applications:

  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at room temperature.

Notes

The molecular weights for the marker used with this product are: 180, 116, 97, 66, 44, 29, 18, 14, 6.

Alternate Names for INSTA-Blot Colon Tissue OncoPair

  • INSTA Blot Colon Tissue OncoPair
  • INSTABlot Colon Tissue OncoPair
  • INSTA-Blot Colon Tissue

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Western Blot Membranes are guaranteed for 1 year from date of receipt.
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Publications for INSTA-Blot Colon Tissue OncoPair (NBP2-30129)(3)

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Other Available Formats

NBP2-30129

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