INSTA-Blot Breast Tissue OncoPair

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Summary

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INSTA-Blot Breast Tissue OncoPair Summary

Description
Tissue
Lane Tissue Type Cat. No. Grade Stage Sex Age Diagnosis
MWM
2 Breast NBP2-27882 2 IIIB F 36 Ductal carcinoma
3 Breast NBP2-27880 Normal adjacent
4 Breast NBP2-27886 2 IV F 61 Ductal carcinoma
5 Breast NBP2-27885 Normal adjacent
6 Breast NBP2-27915 2 IIIB F 54 Ductal carcinoma
7 Breast NBP2-27914 Normal adjacent
8 Breast NBP2-27896 2 IV F 45 Ductal carcinoma
9 Breast NBP2-27894 Normal adjacent
10 Breast NBP2-27928 2 IIIB F 55  Ductal carcinoma
11 Breast NBP2-27927 Normal adjacent
12 Breast NBP2-27948. 2 IV F 85 Ductal carcinoma
13 Breast NBP2-27947 Normal adjacent
14 Breast NBP2-28047 2 IIIB F 48 Ductal carcinomaa
15 Breast NBP2-28045 Normal adjacent
Background
The development of biomarkers has enormous potential for revolutionizing the diagnosis and treatment of disease. Western blot analysis is an integral technique for biomarker profiling. While key for biomarker discovery, the existing body of western blot data has primarily been defined from studies of tumor, immortal, and primary cells growing in vitro. Collectively, results obtained over dec...ades have been integral to the dogma that up- and down-regulation of proteins can be leveraged as biomarkers of normal development, homeostasis, and disease. However, data from human tissue-derived products is key for generating biomarker validation data and progressing the most promising biomarker targets towards the clinic. The OncoPair INSTA-Blot™ product line addresses the increasing demand for the inclusion of human tissue-derived products in biomarker profiling. The OncoPair INSTA-Blot™ is a ready-to-use PVDF western blot membrane containing denatured protein lysates manufactured from Novus's extensive Human Clinical Tissue Lysate collection. Each blot contains 14 lanes of alternating tumor (T) and matched normal adjacent (NA) tissue lysates from 7 patient donors. Incorporating both T and NA on the blot enables protein expression analysis of the tumor and microenvironment. The tumor microenvironment is increasingly being recognized as a major factor in influencing malignant progression and metastatic potential. Cancer is a heterogeneous disease and the presence of multiple donors on a single blot enables rapid screening of protein expression variability between T/NA from different individuals and at different disease stages. Clinical diagnosis and histopathology reports from board-certified pathologists are available for every patient sample. OncoPair INSTA-Blots™ are available for a wide range of tumor types and stages. The product line is manufactured from donor patient tissue obtained from established hospital-based collection sites around the world. Tissues are collected under strict bioethical standards using IRB and HIPAA approved protocols. These protocols ensure patient confidentiality, safety, and informed consent. Tissues are flash frozen at collection sites within minutes of removal, maintained in liquid nitrogen and processed into lysates using protocols optimized for extracting proteins from tissues. Lysates are denatured for OncoPair INSTA-Blot™ production; native lysates are available for follow up proteomic studies. OncoPair INSTA-Blots™ and lysates are also useful in conjunction with the Cell Line and Normal Tissue INSTA-Blots™ and lysates for comprehensive antibody validation and to profile antibody reactivity and protein expression patterns across species and between tissues and cells lines. The OncoPair INSTA-Blots™ are proteomic discovery tools and are useful for screening the expression of various proteins in tumor and normal adjacent tissues from various donors. Researchers may want to follow up on their results by further analysis with the corresponding individual lysates when available.
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Preparation
Method
Tissue specimens were homogenized in modified RIPA buffer to obtain the soluble proteins and centrifuged to clarify. The concentration of protein in each lysate was determined by a standard protein assay and standardized to 1 mg/ml. Sample buffer was added to the soluble fraction and approximately 14 ug/lane of protein was run and then transferred to PVDF membrane. -Soluble fraction extraction buffer (extract 1): PBS at pH 7.4, 1 ug/ml Aprotinin, 1 mM NaF -Modified RIPA buffer: 1 mM EDTA, 1 ug/ml Pepstatin-A, 0.1% SDS , 0.25% Na deoxycholate, 1 ug/ml Leupeptin, 1 mM PMSF, 1 mM Na3VO4 -5X Sample buffer: 50 ml glycerol, 15 g SDS, 3.819 g Tris, pH 6.8, 25 ml 2-ME, 100 mg bromophenol blue, final volume of 100 ml with DI H2O.

Applications/Dilutions

Publications
Read Publications using
NBP2-30126 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at room temperature.

Alternate Names for INSTA-Blot Breast Tissue OncoPair

  • Breast Tissue Onco
  • Breast Tissue OncoPair
  • INSTA Blot
  • INSTA-Blot

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Western Blot Membranes are guaranteed for 1 year from date of receipt.
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Publications for INSTA-Blot Breast Tissue OncoPair (NBP2-30126)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(2)
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Human
(2)
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Showing Publications 1 - 5 of 5.
Publications using NBP2-30126 Applications Species
Santagata S, Hu R, Lin NU et al. High levels of nuclear heat-shock factor 1 (HSF1) are associated with poor prognosis in breast cancer. Proc Natl Acad Sci U S A. 2011 Nov 8 [PMID: 22042860]

Details:
INSTA-BLOT(TM) Breast Tissue OncoPair (IMB-130a). Membrane was probed with HSF1 antibody, Fig 1C.
Tuszynski GP, Rothman VL, Zheng X et al. G-protein coupled receptor-associated sorting protein 1 (GASP-1), a potential biomarker in breast cancer. Exp Mol Pathol. 2011 Oct [PMID: 21791203]

Details:
INSTA-BLOT(TM) Breast Tissue OncoPair (IMB-130e). Blot was probed with a GASP-1 antibody, Fig 2.2. Breast cancer Tissue Microarray (IMH-364, aka CBA4). Tissue microarray was probed with a GASP-1 antibody, Figs 3, 4.
Qin Y, Xu J, Aysola K et al. Ubc9 mediates nuclear localization and growth suppression of BRCA1 and BRCA1a proteins. J Cell Physiol. 2011 Dec [PMID: 21344391]

Details:
INSTA-BLOT(TM) Breast Tissue OncoPair (IMB-130e). Membrane was probed with Ubc9 antibody, Fig 7A.
Michel V, Yuan Z, Ramsubir S et al. Choline transport for phospholipid synthesis. Exp Biol Med (Maywood). 2006 May [PMID: 16636297] (WB, Human)

Details:
Product cited: INSTA-Blot(TM) Human Tissue: ready-to-use WB (IMB-103).
WB Human
Kinouchi R, Kinouchi T, Hamamoto T et al. Distribution of CESP-1 protein in the corneal endothelium and other tissues. Invest Ophthalmol Vis Sci. 2006 Apr [PMID: 16565373] (WB, Human)

Details:
INSTA-BLOT(TM) Human Tissue: ready-to-use WB (IMB-103).
WB Human

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NBP2-30126

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