InnovaCoat® GOLD - 40nm Mini Maleimide Antibody Labeling Kit

Flow Cytometry: InnovaCoat GOLD - Maleimide 40nm Antibody Labeling Kit [270-0005] - Lateral flow assay demonstrates increased performance of orientated (Fab'-InnovaCoat®: GOLD-Maleimide) vs. random (Fab-InnovaCoat®: GOLD) labeling. All components of the lateral flow assay are identical with the exception of the method used to conjugate the Fab' to InnovaCoat®: GOLD-Maleimide or Fab to InnovaCoat®: GOLD. Fab and Fab' fragments were derived from the same antibody.

InnovaCoat GOLD - Maleimide 40nm Antibody Labeling Kit [270-0005]

• Kit Type: Antibody Labeling Kit

InnovaCoat® GOLD - 40nm Mini Maleimide Antibody Labeling Kit Summary

• Description: The InnovaCoat GOLD-Maleimide antibody labeling kit allows thiolated molecules (e.g. antibodies, Fab' fragments, or other sulfhydryl-containing biomolecules) to be covalently attached to ultra-stable InnovaCoat GOLD nanoparticles at very high OD quickly and easily.
  
  The researcher simply has to pipette the solution of the thiol-activated molecule to the vial of InnovaCoat GOLD maleimide. Thee hands-on time for the procedure is about 5 minutes and the conjugate is ready to use within 1 hour.
  
  The InnovaCoat protective coating completely shields the biomolecule from the metal surface thus eliminating metal-antibody interactions and reducing the risk of sub-optimal antibody performance (e.g. loss of affinity or denaturation, especially of small antibody fragments). Moreover, the covalently attached molecule is not prone to dissociation which is a major problem with direct thiol-to-gold metal conjugations.
  
  InnovaCoat GOLD-Maleimide nanoparticles in this kit are supplied as a freeze dried mixture. The conjugation reaction is initiated simply by adding a solution of the thiolated biomolecule, which becomes attached to the InnovaCoat surface by a covalent reaction with maleimide groups.
  
  

  Features	Benefits
  Oriented labeling	Oligonucleotides, and the antigen binding sites of antibodies are free to bind the target molecule
  Proprietary surface coating prevents metal-protein interactions	Covalent bond, stable conjugates formed
  Different nanoparticle sizes and amounts available	Flexible
  Freeze dried	Ships at ambient temperature, long shelf-life
  Fully scalable	Easy transfer from R&D to manufacturing
  Stringently QC tested: uniform spherical shape and narrow size distribution	Consistent high quality, excellent batch-to-batch reproducibility

• Kit Type: Antibody Labeling Kit

Packaging, Storage & Formulations

• Storage: Store at -20C.

Notes

InnovaCoat GOLD® is a registered trademark of Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.