Influenza B Virus Nucleoprotein Antibody (1B6) - BSA Free

Immunocytochemistry/Immunofluorescence: Influenza B Virus Nucleoprotein Antibody (1B6) - BSA Free [NBP1-05187] - diluted 1:500 with a secondary polyclonal rabbit anti-mouse Ig FITC conjugated antibody (DAKO F0232) 1:20 + 1% Evans blue colors Influenza B nucleoprotein. The entire infected Vero cells are visible.

Product Discontinued

Influenza B Virus Nucleoprotein Antibody (1B6) - BSA Free Summary

• Immunogen: Unpurified influenza B virus (B/Lee/40) for primary intranasal immunization, boosted intravenously with purified influenza B virus disrupted with Triton X-100 for 40 min at 37 degrees C.
• Specificity: Reacts with influenza B virus nucleoprotein as demonstrated by immunoblotting after SDS-PAGE. Does not cross-react with influenza A virus (2)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry 1:10-1:500
  • Western Blot 1:40

Reactivity Notes

Virus

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Influenza B Virus Nucleoprotein Antibody (1B6) - BSA Free

• Influenza B Virus NP
• NP
• Nucleoprotein

Background

Influenza viruses are common and highly infectious human pathogens. They are constantly mutating to modify their antigenicity and avoid elimination by immunity to previous generations of virus. The nucleoprotein of influenza B virus is a basic, phosphorylated multimeric protein consisting of 560 aa subunits that encapsidate the viral RNA genome to form a ribonucleoprotein particle (1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.