Influenza B nucleoprotein Antibody (3E9/B2) - (B/Lee/40) - BSA Free

Immunocytochemistry/Immunofluorescence: Influenza B nucleoprotein Antibody (3E9/B2) [NBP2-23513] - BSA Free [NBP2-23513] - diluted 1/1000 with a secondary polyclonal rabbit anti-mouse Ig FITC conjugated antibody 1:20 + 1% Evans blue colors Influenza B nucleoprotein. Primarily the nucleus of the infected Vero cells is visible

Product Discontinued

Influenza B nucleoprotein Antibody (3E9/B2) - (B/Lee/40) - BSA Free Summary

• Immunogen: Unpurified influenza B virus (B/Lee/40) for primary intranasal immunization, boosted intravenously with purified influenza B virus disrupted with Triton X-100 for 40 min at 37C.
• Epitope: Epitope differs from Clone 03 as determined by inhibition ELISA
• Specificity: Influenza B Virus Nucleoprotein (B/Lee/40). This antibody reacts with influenza B virus nucleoprotein as demonstrated by immunoblotting after SDS-PAGE. It does not cross-react with influenza A virus.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunohistochemistry 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Clone 3E9/B2 is suitable as capture antibody in combination with biotinylated Clone 03 as detecting antibody in sandwich ELISA for influenza B virus .Clone 3E9/B2 reacts with influenza B-infected, acetone-fixed VERO cells in immunofluorescence cytochemistry.In Western blotting a dilution guideline of 1/20 has proved successful .

Reactivity Notes

Influenza B virus

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Influenza B nucleoprotein Antibody (3E9/B2) - (B/Lee/40) - BSA Free

• Influenza B Virus NP
• NP
• Nucleoprotein

Background

Influenza virus B is a genus in the virus family Orthomyxoviridae. A limited host range means that Influenza virus B pandemics are rare. The nucleoprotein protects the negative strand viral RNA from nucleases by encapsidating it. encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Influenza B nucleoprotein Antibody (NBP2-23513). (Showing 1 - 1 of 1 FAQ).

1. We ordered your Influenza A nucleoprotein. We tested it. And it worked well for us. I am wondering if you also have Influenza B nucleoprotein, Recombinant. I don’t see it in your catalog.
   • At this time, it appears we only carry antibodies direct towards Influenza B nucleoprotein. We do not currently have a recombinant protein to this target. I apologize for the inconvenience.