Recombinant Virus Influenza A nucleoprotein Protein


ELISA: Recombinant Virus Influenza A nucleoprotein Protein [NBP2-26531] - Influenza A standard curve. Influenza A/NP-A mAb, clone 2F205 was used as the capture antibody. Influenza A/NP-A pAb was used as the detection more
SDS-Page: Recombinant Virus Influenza A nucleoprotein Protein [NBP2-26531] - Coomassie stained SDS-PAGE gel of Influenza A Nuclear Protein: 0.5 ug (lane A), 1.0 ug (lane B), 2.0 ug (Lane C) of protein was loaded and more

Order Details

    • Catalog Number
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

Recombinant Virus Influenza A nucleoprotein Protein Summary

A recombinant protein corresponding to Influenza A nucleoprotein.

Source: Baculovirus expression vector.

Amino Acid Sequence:

A cDNA coding for Human Influenza-A (Influenza A virus (A/Puerto Rico/8/34/Mount Sinai (H1N1)) segment 5) nuclear protein NP was cloned into a Baculovirus expression vector. The recombinant HNP-A was purified by proprietary chromatographic techniques. This protein migrates as a 55 kDa band in SDS-PAGE. The protein concentration was estimated by a BioRad Assay. The purity of the recombinant protein is over 95%.
Sf 21 (baculovirus)
Protein/Peptide Type
Recombinant Protein
>95%, by SDS-PAGE


Application Notes
Use in ELISA and Western blot reported in scientific literature (PMID 25677970)
Read Publications using
NBP2-26531 in the following applications:

  • 1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Lyophilized from 1M sodium chloride.
>95%, by SDS-PAGE
Reconstitution Instructions
Rehydrate with sterile water. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Alternate Names for Recombinant Virus Influenza A nucleoprotein Protein

  • Influenza A virus NP
  • NP
  • NP-A
  • Nucleocapsid protein
  • Nucleoprotein


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.
Supplier Logo

Publications for Influenza A nucleoprotein Recombinant Protein (NBP2-26531)(12)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ELISA, WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NBP2-26531 Applications Species
Nahampun HN, Bosworth B, Cunnick J et al. Expression of H3N2 nucleoprotein in maize seeds and immunogenicity in mice Plant Cell Rep. 2015 Feb 13 [PMID: 25677970] (WB, ELISA) WB, ELISA
Shen X, S0derholm J, Lin F et al. Influenza A vaccines using linear expression cassettes delivered via electroporation afford full protection against challenge in a mouse model. Vaccine. 2012 Nov 6 [PMID: 22406460]

ELISA: The IMR-274 NP-A recombinant protein was used to detect antibody responses in the serum of mice immunized with plasmids encoding NP two weeks following the last immunization (Fig 3a). NP-A (IMR-274) was coated onto a Nunc Maxi-Sorp Immuno Plate at
Buriani G, Mancini C, Benvenuto E, Baschieri S. Heat-shock protein 70 from plant biofactories of recombinant antigens activate multiepitope-targeted immune responses. Plant Biotechnol J. 2012 Apr [PMID: 22221920]

ELISA in plants and mice: 1. Recombinant Influenza A Nucleoprotein (NP-A, IMR-274), Protein standard (Fig 1A) and ELISA capture (Fig 6).
Quinn K, Quirion MR, Lo CY et al. Intranasal administration of adeno-associated virus type 12 (AAV12) leads to transduction of the nasal epithelia and can initiate transgene-specific immune response. Mol Ther. 2011 Nov [PMID: 21829176]

(2011). ELISA: Detection of antibody responses to NP-A after virus vaccination in mice (Fig 5). NP-A (IMR-274) was coated onto ELISA plates at 1 ug/ml in PBS buffer, blocked for 2 h with 1% FBS in PBS and the incubated with fourfold serially diluted serum
Soboleski MR, Gabbard JD, Price GE et al. Cold-adapted influenza and recombinant adenovirus vaccines induce cross-protective immunity against pH1N1 challenge in mice. PLoS One. 2011 [PMID: 21789196]

ELISA: Detection of antibody responses to NP-A in the serum of immunized in mice at various time points following virus immunization (Fig 1). NP-A (IMR-274) was coated onto ELISA plates at 1 ug/ml in 0.125 saline, 0.007 M borate buffer overnight. at 4 deg
Jelinek I, Leonard JN, Price GE et al. TLR3-specific double-stranded RNA oligonucleotide adjuvants induce dendritic cell cross-presentation, CTL responses, and antiviral protection. J Immunol. 2011 Feb 15 [PMID: 21242525]

Immunization (Fig 7): Mice were immunized with 20 ug NP-A (IMR-274) alone or in combination with either dsRNA oligonucleotides (10 ug) or polyinosinic:polycytidylic acid (10 ug) in the rear quadricepts, 7-10 mice/group. Four weeks post immunization, the animal groups were boosted using the same protocol. Three weeks after boosting, the mice were challenged with influenza virus and monitored for survival.
Lin F, Shen X, McCoy JR et al. A novel prototype device for electroporation-enhanced DNA vaccine delivery simultaneously to both skin and muscle. Vaccine. 2011 Sep 9 [PMID: 21199706]
Blitvich BJ, Ibarra-Juarez LA, Cortes-Guzman AJ et al. Seroprevalence of equine influenza virus in north-east and southern Mexico. Vet Rec. 2010 May 1 [PMID: 20435984] (Human)

ELISA, the IMR-274 recombinant protein was used in ELISA to evaluate antibodies to influenza virus sub-types in human serum samples. Data described but not shown.
Rao SS, Kong WP, Wei CJ et al. Comparative efficacy of hemagglutinin, nucleoprotein, and matrix 2 protein gene-based vaccination against H5N1 influenza in mouse and ferret. PLoS One. 2010 Mar 23 [PMID: 20352112]

ELISA: Detection of humoral immune responses to NP after DNA vaccination in mice (Fig 2) or ferrets (Fig 4). The recombinant Influenza A nucleoprotein (NP-A) IMR-274 was used to coat ELISA plates.
Price GE, Soboleski MR, Lo CY et al. Vaccination focusing immunity on conserved antigens protects mice and ferrets against virulent H1N1 and H5N1 influenza A viruses. Vaccine. 2009 Nov 5 [PMID: 19729082]

ELISA (Fig 1): Detection of immune responses to NP after influenza A plasmid immunization in mice. NP-A (IMR-274) was used to coat ELISA plates. Antibody levels were measured in serum, nasal wash and brochoalveolar lavage. Data was expressed as endpoint titers defined as the highest dilution of sample giving an OD 405 nm reader greater than three SD above the mean of naive samples.
Show All 12 Publications.

Reviews for Influenza A nucleoprotein Recombinant Protein (NBP2-26531) (0)

There are no reviews for Influenza A nucleoprotein Recombinant Protein (NBP2-26531). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Influenza A nucleoprotein Recombinant Protein (NBP2-26531). (Showing 1 - 1 of 1 FAQs).

  1. For the product #NBP2-26531 (Influenza A nucleo-protein), could you let us know the buffer (solvent) composition? Is it no preservative?
    • Our Influenza A nucleoprotein with catalogue number NBP2-26531 is lyophilized from 1M sodium chloride. The protein should be reconstituted with sterile water. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA), and to avoid freeze-thaw cycles.

Additional Influenza A nucleoprotein Products

Influenza A nucleoprotein NBP2-26531

Blogs on Influenza A nucleoprotein

There are no specific blogs for Influenza A nucleoprotein, but you can read our latest blog posts.

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Virus Influenza A nucleoprotein Protein and receive a gift card or discount.