Recombinant Virus Influenza A nucleoprotein Protein

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ELISA: Influenza A nucleoprotein Protein [NBP2-24986] - Influenza A/NP-A mAb, clone 2F205 was used as the capture antibody. Influenza A/NP-A pAb was used as the detection antibody. Recombinant Influenza A Nucleoprotein ...read more
SDS-Page: Influenza A nucleoprotein Protein [NBP2-24986] - 2 ug (lane A), 5 ug (lane B), 10 ug (Lane C) of protein was loaded and separated by 4-20% SDS-PAGE.

Product Details

Summary
Reactivity ViSpecies Glossary
Applications ELISA, IHC-P, PAGE
Concentration
1 mg/ml

Order Details

Recombinant Virus Influenza A nucleoprotein Protein Summary

Preparation
Method
A cDNA coding for Human Influenza-A (Influenza A virus (A/Puerto Rico/8/34/Mount Sinai (H1N1)) segment 5) nuclear protein NP was cloned into a Baculovirus expression vector. The recombinant HNP-A was purified by proprietary chromatographic techniques.
Protein/Peptide Type
Recombinant Protein
Purity
Chromatography

Applications/Dilutions

Dilutions
  • SDS-Page 2-10 ug/lane
Application Notes
This protein migrates as a 55 kDa band in SDS-PAGE. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24657788). The protein concentration was estimated in a BioRad assay. The absolute purity of the recombinant protein preparation has not been established.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-24986 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
20 mM HEPES, pH7.0, 1.5 mM MgCl2, 0.2 mM EDTA, 0.5 mM PMSF, and 0.5 mM DTT.
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Chromatography

Notes

For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Alternate Names for Recombinant Virus Influenza A nucleoprotein Protein

  • Influenza A virus NP
  • NP
  • NP-A
  • Nucleocapsid protein
  • Nucleoprotein

Background

This protein migrates as a 55 kDa band in SDS-PAGE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.
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Publications for Influenza A nucleoprotein Recombinant Protein (NBP2-24986)(23)

We have publications tested in 3 confirmed species: Human, Mouse, Bovine.

We have publications tested in 5 applications: ELISA, IHC-P, In vitro, In vivo, in-vitro.


Filter By Application
ELISA
(6)
IHC-P
(1)
In vitro
(1)
In vivo
(1)
in-vitro
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(2)
Bovine
(1)
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using NBP2-24986 Applications Species
Singh S, Nehete PN, Yang G et al. Enhancement of Mucosal Immunogenicity of Viral Vectored Vaccines by the NKT Cell Agonist Alpha-Galactosylceramide as Adjuvant. Vaccines. 2014 Oct 10 [PMID: 25553254] (In vitro, In vivo)

Details:
Influenza A nucleoprotein Protein (NP) used for in vitro and in vivo experiments involving Rhesus Macaques / Monkeys model.
In vitro, In vivo
Panyasing Y, Goodell C, Kittawornrat A et al. Influenza A Virus Surveillance Based on Pre-Weaning Piglet Oral Fluid Samples. Transbound Emerg Dis. 2014 Dec 9 [PMID: 25488821]
He XS, Holmes TH, Sanyal M et al. Distinct patterns of B-cell activation and priming by natural influenza infection versus inactivated influenza vaccination. J. Infect. Dis. 2014 Oct 21 [PMID: 25336731] (in-vitro) in-vitro
Yeo Sj, Huong Dt, Hong Nn et al. Rapid and Quantitative Detection of Zoonotic Influenza A Virus Infection Utilizing Coumarin-derived dendrimer-based Fluorescent Immunochromatographic Strip Test (FICT). Theranostics. 2014 Oct 06 [PMID: 25285172]
Luetkens T, Kobold S, Cao Y et al. Functional autoantibodies against SSX-2 and NY-ESO-1 in multiple myeloma patients after allogeneic stem cell transplantation. Cancer Immunol. Immunother. 2014 Jul 31 [PMID: 25078248]
Fontana JM, Christos PJ, Michelini Z et al. Mucosal Immunization with Integrase-Defective Lentiviral Vectors Protects against Influenza Virus Challenge in Mice. PLoS ONE. 2014 May 14 [PMID: 24824623] (ELISA, Mouse)

Details:
ELISA: Mouse serum from mice innoculated with IDLV-NP (lentiviral vector expression the influenza virus nucleoprotein), Figs 4, 5. The Influenza A recombinant nucleoprotein was used for an NP-specific serum IgG ELISA. 96-well microplates were coated with
ELISA Mouse
Sasaki S, Holmes TH, Albrecht RA et al. Distinct cross-reactive B-cell responses to live attenuated and inactivated influenza vaccines. J. Infect. Dis. 2014 Mar 27 [PMID: 24676204] (ELISA, Human)

Details:
Fig 7A, ELISPOT plates were coated with the recombinant influenza A virus nuclear protein at 4.5 ug/ml.
ELISA Human
Kwon D, Shin K, Kim SJ et al. Mammalian pathogenesis of oseltamivir-resistant pandemic (H1N1) 2009 influenza virus isolated in South Korea. Virus Res. 3/19/2014 [PMID: 24657788] (IHC-P) IHC-P
Panyasing Y, Goodell CK, Gimenez-Lirola L et al. Kinetics of influenza A virus nucleoprotein antibody (IgM, IgA, and IgG) in serum and oral fluid specimens from pigs infected under experimental conditions. Vaccine 2013 Dec 16 [PMID: 24200976] (ELISA) ELISA
Lin Feng, Shen Xuefei, Kichaev Gleb et al. Optimization of electroporation-enhanced intradermal delivery of DNA vaccine using a minimally invasive surface device. Hum Gene Ther Methods. 2012 Jun [PMID: 22794496] (ELISA, Mouse) ELISA Mouse
Show All 23 Publications.

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