Influenza A nucleoprotein Antibody (5D8) - BSA Free

Immunocytochemistry/Immunofluorescence: Influenza A nucleoprotein Antibody (5D8) [NBP2-23568] - diluted 1:1000 + secondary polyclonal rabbit anti-mouse Ig FITC conjugated antibody 1:20 + 1% Evans blue colors Influenza A nucleoprotein.Influenza Virus slides (S6112) were used and the entire infected Vero cells are seen.

Immunocytochemistry/Immunofluorescence: Influenza A nucleoprotein Antibody (5D8) [NBP2-23568] - diluted 1:1500 + secondary polyclonal goat anti-mouse Ig Fluor 555 conjugated antibody (Alexa A21424) 1:200 + Vectashield mounting medium with DAPI. Influenza Virus slides (S6112) were used and the entire infected Vero cells are seen.

Product Discontinued

Influenza A nucleoprotein Antibody (5D8) - BSA Free Summary

• Immunogen: Unpurified influenza A virus (H1N1 A/PR/8/34) for primary intranasal immunization, boosted intravenously with purified influenza A virus disrupted with Triton X-100 for 45 min at 37C.
• Epitope: Not determined
• Specificity: Clone 5D8 is specific for influenza A virus nucleoprotein and does not cross-react with influenza B virus.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: Clone 5D8 is suitable as capture antibody in a sandwich ELISA for influenza A virus, using Clone 5D8B (biotinylated) for detection .Clone 5D8 reacts with influenza A-infected, acetone-fixed VERO cells in immunofluorescence cytochemistry..

Reactivity Notes

Influenza A virus

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Influenza A nucleoprotein Antibody (5D8) - BSA Free

• Influenza A virus NP
• NP
• NP-A
• Nucleocapsid protein
• Nucleoprotein

Background

IInfluenza viruses are common and highly infectious human pathogens. They are constantly mutating to modify their antigenicity and avoid elimination by immunity to previous generations of virus. The nucleoprotein of influenza A virus is a basic, phosphorylated multimeric protein consisting of 498 aa subunits that encapsidate the viral RNA genome to form a ribonucleoprotein particle

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.