Influenza A Hemagglutinin H1 Antibody (InA4)

Western Blot: Influenza A Haemagglutinin H1 Antibody (InA4) [NB100-73155] - Immunodetection of Influenza A viruses using anti-haemagglutinin monoclonal antibodies in Western blotting after PAGE in reducing conditions. Anti-mouse IgG conjugated with HRP was used for MAb haemagglutinin complex visualization. Antigens (1ug/well): H1N1 - Influenza A/New Caledonia/20/99 H3N2 - Influenza A/Shangdong/9/93 Antibodies (5ug/ml): 1: MAb InA4 - anti-Influenza A haemagglutinin H1

Product Discontinued

Influenza A Hemagglutinin H1 Antibody (InA4) Summary

• Immunogen: Influenza A/NewCaledonia/20/99 (strain H1N1) derived from allantoic fluid of 10 days old embryonated eggs.
• Specificity: Influenza A haemagglutinin H1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoassay
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: Recommended pairs for Influenza A H1 in sandwich immunoassay (capture-detection): NB100-73155 - NB100-73159

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Influenza A Hemagglutinin H1 Antibody (InA4)

• HA
• Hemagglutinin HA
• Hemagglutinin

Background

Influenza A virus is a major public health threat. Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals; however it is in birds that all subtypes can be found. These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.