Recombinant Influenza A H3N2/HA His Protein

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SDS-Page: Recombinant Virus Influenza A H3N2/HA Protein [NBP1-99037]

Product Details

Summary
Applications ELISA, PAGE
Concentration
1 mg/ml

Recombinant Influenza A H3N2/HA His Protein Summary

Description
A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 18-344 of H3N2/HA (Canine) Influenza A H3N2

Source: E.coli

Amino Acid Sequence: ADPNLPGNENN AATLCLGHHA VPNGTIVKTI TDDQIEVTNA TELVQNSSTG KICNNPHKIL DGRDCTLIDA LLGDPHCDVF QNETWDLFVE RSNAFSNCYP YDVPDYASLR SIVASSGTLE FITEGFTWAG VTQNGGSGAC KKGPANGFFS RLNWLTKSGN TYPVLNVTMP NNNNFDKLYI WGVHHPSTNQ EQTSLYIQAS GRVKVSTRRS QQTIIPNIGS RPLVRGQSGR ISVYWTIVKP GDVLVINSNG NLIAPRGYFK MRIGKSSIMR SDAPIDTCIS ECITPNGSIP NEKPFQNVNK ITYGACPKYV KQNTLKLATG MRNVPERQTH HHHHH

Source
Baculovirus
Protein/Peptide Type
Recombinant Protein
Purity
>95% pure by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1ug of protein (determined by LAL method)

Applications/Dilutions

Application Notes
Use in ELISA reported in secitific publication PMID: 32849530
Theoretical MW
36.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-99037.

Reactivity Notes

Influenza A Virus H3N2 (canine)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Background

Influenza A virus subtype H3N2, also known as H3N2, is a subtype of viruses that cause influenza (flu). H3N2 Viruses can infect birds and mammals. In birds, humans, and pigs, the virus has mutated into many strains. Hemagglutinin(HA) binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. It plays a major role in the determination of host range restriction and virulence and is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Recombinant Influenza A virus (H3N2, canine) HA protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Influenza A H3N2/HA Recombinant Protein (NBP1-99037)(1)

We have publications tested in 1 application: ELISA.


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FAQs for Influenza A H3N2/HA Recombinant Protein (NBP1-99037). (Showing 1 - 1 of 1 FAQ).

  1. Does the protein NBP1-99037 have infectivity?
    • No, NBP1-99037 is not infectious. The purpose of manufacturing this product by our lab is for using as the positive control in WB/PAGE.  NBP1-99037 is a recombinant protein, containing mainly the amino acids 18-344 derived from the hemagglutinin (HA) of the Influenza A H3N2 (canine) strain.  Although HA has been know to bind to the receptor on the cell surface, the carbohydrate moiety is responsible for interaction.  Fortunately, the E. coli-expressed proteins won't have the glycosylated modifications, hence this protein is not going to bind to influenza's receptor. In summary, this is just a regular recombinant protein, similar to many others, that do not post any harmful effect of any kind to human.

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