Influenza A H1N1 Hemagglutinin ELISA Kit (Colorimetric)

ELISA: Influenza A Haemagglutinin H1N1 ELISA Kit (Colorimetric) [NBP2-80333] - Example standard curve for Influenza A Haemagglutinin H1N1 ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ELISA: Influenza A Haemagglutinin H1N1 ELISA Kit (Colorimetric) [NBP2-80333] - This assay recognizes most of recombinant H1N1 Hemagglutinin protein. With influenza A H1N1 Hemagglutinin - A/California/04/2009 as the standard, reaction of other H1N1 strains were as follows. The representative strains of other subtypes listed in the below table were prepared at 50 ng/mL in dilution buffer and assayed where no cross-reactivity was observed. Cross-reactivity was observed to be 1.13% with 50 ng/mL of recombinant influenza A H2N2 Hemagglutinin - A/Canada/720/2005 Protein

Product Discontinued

Influenza A H1N1 Hemagglutinin ELISA Kit (Colorimetric) Summary

• Specificity: Recognizes both recombinant and natural Influenza A Haemagglutinin H1N1.
• Standard Curve Range: 31.25-2000 pg/ml (example only; lot dependent)
• Sensitivity: 14.87 pg/ml
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 8.9 %
• Intra-Assay: CV% < 2.4
• Spike Recovery: serum (n=3): 81 -87%
  supernatant (n=3): 92 -111%
  urine (n=3): 81 -87%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage is content dependent.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Review for Influenza A H1N1 Hemagglutinin ELISA Kit (NBP2-80333) (1)

Average Rating: 4

(Based on 1 review)

reviewed by: Chenxu Guo 08/28/2023

Summary

• Sample Tested: Influenza A H1N1 HA vaccine
• Lot: CW16MY2503US2

FAQs for Influenza A H1N1 Hemagglutinin ELISA Kit (NBP2-80333). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.