Human Influenza A Antibody (IgM) ELISA Kit (Colorimetric) Summary
Description |
Assay Length: 1-5 hours |
Assay Type |
Indirect ELISA |
Inter-Assay |
%CV < 15 |
Intra-Assay |
%CV < 15 |
Sample Volume |
50 - 100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Adhesive Strip
- Coated assay plate
- HRP-conjugate
- Instruction manual
- Negative Control
- Positive Control
- Sample Diluent
- Stop Solution
- Substrate A
- Substrate B
- Wash Buffer (25 x concentrate)
|
Background
This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Ag. Samples are pipetted into the wells with anti-human IgM conjugated Horseradish Peroxidase (HRP). Any antibody specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human influenza A virus (FluA) antibody (IgM) bound in the intial step. The color development is stopped and the intensity of the color is measured.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Influenza A Antibody (IgM) ELISA Kit (NBP2-60070). (Showing 1 - 1 of 1 FAQ).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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