Infectious Bronchitis Virus Antibody (IB95) - BSA Free

• Reactivity: V-Vi
• Applications: WB, ELISA, ICC/IF
• Clone: IB95
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Infectious Bronchitis Virus Antibody (IB95) - BSA Free Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with semi-purified inactivated Infectious Bronchitis virus of Massachusetts type from infected chicken embryos.
• Specificity: Nucleoprotein of IBV
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot
• Application Notes: Immunodetection of IBV N-protein in Western Blotting; IB virus detection in sandwich-type immunoassay (capture). Use in ICC/IF reported in scientific literature (PMID:33472590)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Infectious Bronchitis Virus Antibody (NBP2-31102)(7)

Publications using NBP2-31102

• Isham I, Abd-Elsalam R, Mahmoud M et al. Comparison of Infectious Bronchitis Virus (IBV) Pathogenesis and Host Responses in Young Male and Female Chickens Viruses 2023-11-22 [PMID: 38140526] (IHC-P)
• Isham IM, Hassan MSH, Abd-Elsalam RM et al. Impact of Maternal Antibodies on Infectious Bronchitis Virus (IBV) Infection in Primary and Secondary Lymphoid Organs of Chickens Vaccines 2023-07-07 [PMID: 37515032] (IHC-P)
• Hassan MSH, Abd-Elsalam RM, Ratcliff N et al. The impact of the experimental route of challenge on the host responses and pathogenesis of the Canadian Delmarva (DMV/1639) infectious bronchitis virus infection in laying chickens Veterinary immunology and immunopathology 2023-06-22 [PMID: 37364440] (IHC-P)
• Li H, Ni R, Wang K et al. Chicken interferon-induced transmembrane protein 1 promotes replication of coronavirus infectious bronchitis virus in a cell-specific manner Veterinary Microbiology 2022-10-01 [PMID: 36368134] (ICC/IF, Virus)
• Najimudeen S, Barboza-Solis C, Ali A Et al. Pathogenesis and host responses in lungs and kidneys following Canadian 4/91 infectious bronchitis virus (IBV) infection in chickens Virology 2021-12-01 [PMID: 34890893] (IHC-Fr, Virus)
• Li H, Cui P, Fu X, et al. Identification and analysis of long non-coding RNAs and mRNAs in chicken macrophages infected with avian infectious bronchitis coronavirus BMC genomics 2021-01-20 [PMID: 33472590] (ICC/IF)
• Li H, Li J, Zhai Y et al. Gga-miR-30d regulates infectious bronchitis virus infection by targeting USP47 in HD11 cells Microb. Pathog. 2020-01-23 [PMID: 31982568]

FAQs for Infectious Bronchitis Virus Antibody (NBP2-31102). (Showing 1 - 1 of 1 FAQs).

1. What is the concentration of this 1mg preparation?
   • The concentration of this product varies from lot to lot. The lot specific concentration will be written in the vial label as well as the data sheet which is shipped together with each vial. For example for the current lot of #3BN1-IB95, the concentration is 6.0 mg/ml. The purity of all our antibodies in general is >92%