Indole-3-butyric acid Antibody - BSA Free

• Reactivity: All-NA
• Applications: ELISA, AP
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated
• Format: BSA Free

Indole-3-butyric acid Antibody - BSA Free Summary

• Description: Novus Biologicals Chicken Indole-3-butyric acid Antibody - BSA Free (NBP2-88846) is a polyclonal antibody validated for use in ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: BSA-Indole-3-butyric acid conjugate
• Specificity: Target: Indole-3-butyric acid (IBA), CAS no.: 133-32-4, Solubility target: Methanol, ethanol.
  
  Cross Reactivity
  
  Indole-3-actic acid: < 0.1 %
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Purity: PEG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
• Application Notes: The antibody may be used in ELISA and Immuno-affinity-chromatography (IAC). Samples may include cutting medium samples and extraction of these samples may be performed using PBS-10% methanol, followed by neutralization of the extract. Suggested dilution 1:12,800 in indirect ELISA (this titer is defined as the dilution that gives 50 % of the absorbance from the maximum absorbance when tested with ELISA). Plates are coated with 400 ng/ml ovalbumin-conjugated indole-3-butyric acid. HRP-conjugated anti-chicken IgY should be used as a tracer in a 1:8,000 dilution.
  
  Immuno-affinity-chromatography.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 M PBS (pH 7.2)
• Preservative: 0.02% Sodium Azide
• Purity: PEG purified

Notes

Chicken products cannot be exported to Canada.

Store at 4C or -20 C. The working antibody solution is stable for at least 7 days at 4C. Precautions should be taken for storage for longer periods. Problems of long-term stability may occur with highly diluted solutions.
For long storage purposes in solution the addition of sodium azide to 0.02 % is advised with the appropriate precautions of use.

Alternate Names for Indole-3-butyric acid Antibody - BSA Free

• 3-Indolebutyric acid
• IBA

Background

Indole-3-butyric acid (IBA) is a plant hormone belonging to the group of auxins. It is used to stimulate root formation in plant cuttings.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.