Immunoplate for glial derived exosome isolation (Colorimetric)

Immunoplate for glial derived exosome isolation (Colorimetric) [NBP3-14802] - CD63 profiling on exosomes derived from supernatants of different cell lines.

Immunoplate for glial derived exosome isolation (Colorimetric) [NBP3-14802] - Comparison of CD9 detection on purified (ultracentrifuged) plasma exosomes vs unfractionated samples in a set of healthy donor’s plasma.

Immunoplate for glial derived exosome isolation (Colorimetric) [NBP3-14802] - Common exosomal biomarkers analysis in a healthy donor’s plasma sample.

Immunoplate for glial derived exosome isolation (Colorimetric) [NBP3-14802] - Plate is selective in capturing purified exosomes (pellet after centrifugation 120000g) and no other circulating microvesicle (pellet 1200g and 10000g).

Immunoplate for glial derived exosome isolation (Colorimetric) [NBP3-14802] - Specific immunocapture of 30 μg of exosome isolated from Neuroblastoma (SH-30) or Glioblastoma (U87-30) cell lines in specific immunoplates. COLO cell and plasma (PEP) purified exosomes were used as controls.

Immunoplate for glial derived exosome isolation (Colorimetric) [NBP3-14802] - Immunocapture of glioblastoma derived exosomes (U87) subpopulation diluted in human plasma from healthy donors.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Human Plasma

Immunoplate for glial derived exosome isolation (Colorimetric) Summary

• Description: Immunoplates coated with Myelin PLP antibody allow for enrichment of glial derived exosomes from human plasma samples.
  
  Immunoplates provide flexibility for optimization of sandwhich ELISA assays with additional detection antibody, as well as other downstream applications like RNA extraction.
  
  Expiration date is 12 months. Stored up to 24 months (if unopened). After opening, buffers and solutions are good for 1 month, plate strips are good for 6 months. After reconstitution, standards can be stored at -20C for 1 month, -80C for 6 months.
• Sample Volume: 100 uL

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoassay

Packaging, Storage & Formulations

• Storage: Store at 4C.

Background

Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both the amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, and virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (broncho alveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and have an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.