ImmPACT™ VECTOR® RED AP Substrate

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Product Details

Summary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, ISH, ESPOT

Order Details

ImmPACT™ VECTOR® RED AP Substrate Summary

Description
Features:

  • The most sensitive red AP substrate
  • 2-4 fold greater sensitivity than original substrate kit
  • Ideal for IHC/ICC/ISH and blots
  • Fluorescent
  • Heat Stable
  • Permanent mounting
  • Ideal for single and multiple labeling
  • One year expiry date
  • Supplied as 120 ml of ready-to-use diluent and stock solutions in dropper bottles
  • Excitation: broad excitation spectrum, approx. 365 nm to 560 nm
  • Emission: Above 560 nm
  • Color: Red
  • Applications/Dilutions

    Application Notes
    This product consists of 120 mL of ready-to-use diluent and concentrated stock solutions in convenient dropper bottles. Both formulations can be used for either manual or automated staining methods. Either substrate formulation can also be used on blots.

    Packaging, Storage & Formulations

    Storage
    Store at 4C in the dark.

    Kit Components

    Components
    1. ImmPACT™ Vector® Red Diluent (120 mL)
    2. ImmPACT™ Vector® Red Reagent 1 (2.5 mL)
    3. ImmPACT™ Vector® Red Reagent 2 - Tween® 20 (2.5 mL)

    Notes

    This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

    Background

    Preparations stained with either Vector(R) Red or ImmPACT(TM) Vector(R) Red AP chromogen can be dehydrated, cleared, and permanently mounted. Both chromogen formulations can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications. Because of their stability under ISH conditions, both chromogens can be used in IHC/ISH double labeling applications. With the aid of imaging systems and software, the spectral profile of Vector(R) Red or ImmPACT(TM) Vector(R) Red substrate can be distinguished from other enzyme substrates in applications where antigens are co-localized. Both Vector(R) Red and ImmPACT(TM) Vector(R) Red alkaline phosphatase (AP) substrates produce red, heat stable reaction products that are also highly fluorescent and non-fading. The bright fluorescence of the Vector(R) Red or ImmPACT(TM) Vector(R) Red reaction products are visible using DyLight(R) 594, Texas Red(R) or TRITC filter systems. The fluorescence is especially bright in dehydrated, permanently mounted sections. Compared to other AP substrates, the Vector(R) Red and ImmPACT(TM) Vector(R) Red reaction products have a wider dynamic range, offering better quantitation of targets on blots.

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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    Product General Protocols

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    Video Protocols

    WB Video Protocol
    ICC/IF Video Protocol

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