ImmPACT™ NovaRED™ HRP Substrate

• Applications: WB, Func, IHC, ELISA, ICC/IF

ImmPACT™ NovaRED™ HRP Substrate Summary

• Description: Features:
  
• 2-4 fold greater sensitivity than original substrate kit
• Optimized for IHC/ICC/ISH and blots
• Permanent mounting
• Ideal for single and multiple labeling
• Working solutions stable for up to 14 days at 4C
• One year expiry date
• Supplied as 120 ml of diluent and stock solutions in dropper bottles

Applications/Dilutions

• Dilutions:
  • Enzyme-linked Immunosorbent Spot
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • In-situ Hybridization
  • Western Blot
• Application Notes: Sections stained with either substrate should be dehydrated, cleared, and permanently mounted. Sections stained with either substrate can also be viewed by darkfield microscopy. This product consists of 120 ml of diluent and concentrated stock solutions of ImmPACT(TM) NovaRED(TM) reagents in convenient dropper bottles. This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes. Working solutions are stable for up to 14 days at 4C..

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

Vector(R) NovaRED(TM) and ImmPACT(TM) NovaRED HRP substrates produce red reaction products. Both chromogens are useful as alternatives to DAB or as a second color for multiple antigen labeling. Vector(R) NovaRED(TM) and ImmPACT(TM) NovaRED(TM) substrates also provide excellent color contrast in pigmented tissue such as melanoma. With the aid of imaging systems and software, the spectral profile of both substrates can be distinguished from other enzyme substrates in applications where antigens are co-localized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.