ImmPACT™ DAB HRP Substrate


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Product Details

Applications WB, IHC, IHC-Fr, IHC-P, ISH, ESPOT, ICC/IF

Order Details

ImmPACT™ DAB HRP Substrate Summary

  • 2-4 fold greater sensitivity than original substrate kit
  • Optimized for IHC/ICC/ISH and blots
  • Heat Stable
  • Permanent mounting
  • Ideal for single and multiple labeling
  • Working solutions stable for up to 14 days at 4C
  • One year expiry date
  • Supplied as 120 ml of diluent and concentrated DAB stock solution

  • Applications/Dilutions

    • Immunocytochemistry
    • Immunohistochemistry
    • Immunohistochemistry-Frozen
    • Immunohistochemistry-Paraffin
    • In-situ Hybridization
    • Western Blot
    Application Notes
    This product is an entirely new formula for a diaminobenzidine based peroxidase substrate. ImmPACT(TM) DAB produces a dark brown reaction product that is crisper and is 3-4 times more sensitive than the original Vector DAB substrate kit. It can be used for both manual and automated staining methods. Sections stained with DAB can also be viewed by darkfield and electron microscopy. Slides developed with DAB can be dehydrated, cleared, and permanently mounted. The ImmPACT(TM) DAB working solution is stable for at least five days at room temperature and at least two weeks when stored at 4C. This product consists of 120 ml of diluent and a convenient concentrated stock solution of highly purified DAB. This product can also be used on blots.

    Packaging, Storage & Formulations

    Store at 4C in the dark.

    Kit Components

    1. ImmPACT™ DAB Chromogen concentrate (3.6 mL)
    2. ImmPACT™ DAB Diluent (120 mL)


    This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.


    DAB (3,3-diaminobenzidine) HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications. DAB chromogen is effective as a single label or as a second color for multiple antigen labeling. Because of its heat-resistance, DAB can be used in IHC/ ISH double labeling applications. With the aid of imaging systems and software, the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co-localized.


    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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    Product General Protocols

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    Video Protocols

    WB Video Protocol

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