ImmPACT™ DAB EqV HRP Substrate

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Product Details

Summary
Applications WB, IHC, IHC-Fr, IHC-P, ISH, ESPOT, ICC/IF

Order Details

ImmPACT™ DAB EqV HRP Substrate Summary

Description
Features:
  • Optimized for IHC/ICC/ISH
  • Suitable for single and multiple labeling
  • Heat stable
  • Permanent mounting
  • Ideal for single and multiple labeling
  • Can be used for blot detection on nylon and PVDF membranes but is not recommended for nitrocellulose membranes
  • Working solution stable for several days at 4C
  • One year expiry date
  • Consists of 200 mL each of Reagent 1 and Reagent 2 which when mixed together in equal volumes form the working solution

  • Applications/Dilutions

    Application Notes
    ImmPACT(TM) DAB EqV (equal volume) consists of 200 ml each of Reagent 1 and Reagent 2 which when mixed together in equal volumes form the working solution. ImmPACT(TM) DAB EqV produces a dark brown reaction product that is 3 to 5 times more sensitive than the original DAB substrate. The working solution is stable for several days when stored at 4C. This substrate can be used for both manual and automated staining methods. ImmPACT(TM) DAB EqV can be used for blot detection on nylon and PVDF membranes but is not recommended for nitrocellulose membranes. Sections stained with DAB can also be viewed by darkfield and electron microscopy. Slides developed with DAB can be dehydrated, cleared, and permanently mounted.

    Packaging, Storage & Formulations

    Storage
    Store at 4C in the dark.

    Kit Components

    Components
    1. 200 ml ImmPACT™ DAB EqV Reagent 1
    2. 200 ml ImmPACT™ DAB EqV Reagent 2

    Notes

    This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

    Background

    DAB (3,3-diaminobenzidine) HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications. DAB chromogen is effective as a single label or as a second color for multiple antigen labeling. Because of its heat-resistance, DAB can be used in IHC/ISH double labeling applications. With the aid of imaging systems and software, the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co-localized.

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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