ImmPACT™ DAB EqV HRP Substrate

• Applications: WB, Func, IHC, ELISA, ICC/IF

ImmPACT™ DAB EqV HRP Substrate Summary

• Description: Features:
  
• Optimized for IHC/ICC/ISH
• Suitable for single and multiple labeling
• Heat stable
• Permanent mounting
• Ideal for single and multiple labeling
• Can be used for blot detection on nylon and PVDF membranes but is not recommended for nitrocellulose membranes
• Working solution stable for several days at 4C
• One year expiry date
• Consists of 200 mL each of Reagent 1 and Reagent 2 which when mixed together in equal volumes form the working solution

Applications/Dilutions

• Dilutions:
  • Enzyme-linked Immunosorbent Spot
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • In-situ Hybridization
  • Western Blot
• Application Notes: ImmPACT(TM) DAB EqV (equal volume) consists of 200 ml each of Reagent 1 and Reagent 2 which when mixed together in equal volumes form the working solution. ImmPACT(TM) DAB EqV produces a dark brown reaction product that is 3 to 5 times more sensitive than the original DAB substrate. The working solution is stable for several days when stored at 4C. This substrate can be used for both manual and automated staining methods. ImmPACT(TM) DAB EqV can be used for blot detection on nylon and PVDF membranes but is not recommended for nitrocellulose membranes. Sections stained with DAB can also be viewed by darkfield and electron microscopy. Slides developed with DAB can be dehydrated, cleared, and permanently mounted..

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

DAB (3,3-diaminobenzidine) HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications. DAB chromogen is effective as a single label or as a second color for multiple antigen labeling. Because of its heat-resistance, DAB can be used in IHC/ISH double labeling applications. With the aid of imaging systems and software, the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co-localized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.